产品名称
Neutralizing Buffer, for Northern and Southern blotting, powder
grade
Molecular Biology
form
powder
technique(s)
Southern blotting: suitable
foreign activity
DNase, RNase, none detected
storage temp.
room temp
Quality Level
Application
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
General description
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
Preparation Note
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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