usage
sufficient for 10 preparations
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA extraction: suitable
test parameters
sample volume: 150 mL bacterial culture
greener alternative category
storage temp.
15-25°C
General description
GenElute HP 无内毒素质粒大量制备试剂盒可以简单、快速地从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。该试剂盒采用了可快速澄清细菌裂解液的滤柱和可捕获质粒DNA的硅胶柱。配套的专利溶液可使质粒DNA结合到结合柱上，同时避免内毒素随之吸附。该技术使得用户可以维持一致的、低于 0.1 EU/μg 质粒DNA的内毒素水平。
制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。
制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。
我们致力于为您带来更加绿色的替代产品，这些产品遵守一项或多项绿色化学12项原则。相比标准DNA提取方法使用苯酚和氯仿，该产品是一种更安全的化学产品。
Biochem/physiol Actions
通过离心分离法收集过夜的重组大肠杆菌培养液，然后通过改性SDS 碱裂解法进行处理。过滤澄清裂解液，然后加入针对无内毒素质粒制备而优化的结合溶液。随后，质粒捕获到硅胶膜上，同时避免内毒素随之吸附到膜上。通过两遍洗涤步骤，去除污染物。最后，在不含内毒素的水中洗脱结合所得的DNA。回收的质粒DNA主要以超螺旋形式存在。所含的基因组DNA和RNA均低于溴化丙啶染色琼脂糖凝胶电泳的检测水平。
Features and Benefits
- 可纯化出多达1.2 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
- 从沉淀细胞到获得纯化的质粒，仅需35分钟
- 方便的真空形式
Other Notes
更多信息，请见www.sigma-aldrich.com/genelutehp。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
存储类别
10 - Combustible liquids
法规信息
低风险生物材料
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NA0400-1KT | 04061835573691 |