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Merck
CN

NA2010

Sigma-Aldrich

GenElute™ 血液基因组DNA试剂盒

sufficient for 70 purifications

别名:

血液基因组DNA, Gen Elute

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关于此项目

UNSPSC代码:
41105501
NACRES:
NA.55

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用途

sufficient for 70 purifications

质量水平

200

技术

DNA purification: suitable

储存温度

15-25°C
room temp

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相关类别

一般描述

GenElute血液基因组DNA试剂盒可以简单、方便地从新鲜或长时间存放（超过24小时）的全血中分离高纯度基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
Click here to view the kit protocol

应用

GenElute™血液基因组DNA试剂盒已被用于：
  • 从切除输精管的兔子体内获得的大颗粒（LG）和小颗粒（SG）样品中提取DNA
  • 从血液样本中分离DNA
  • 从剩余血细胞中提取全血DNA

纯化所得的基因组DNA可用于以下下游应用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆

生化/生理作用

起始材料首先溶解在含有离液盐的溶液之中，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。随附一份Prewash Solution，可帮助与全血样本长时间存放（超过24小时）相关的污染物。在洗涤去除污染物后，将DNA放在200 mL的Tris-EDTA溶液中洗脱。

预期基因组DNA得率取决于所用的起始材料的数量和性质（例如，从200 μl 新鲜的全血之中，可在一小时内分离出4至10 μg RNase A处理的DNA）。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

特点和优势

  • 起始材料：不超过200 μl新鲜或长时间存放的血液
  • 预期得率：至多10 μg
  • 洗脱体积：400 μl
  • 所需时间：<40分钟
  • A260/A280比率：1.6 - 1.9
  • 兼容多种抗凝血素、包括EDTA、肝素和柠檬酸钠
  • 通常得率为4-10 μg/200 μL全血样本
  • 可获得A260/A280 比率介于1.6-1.9之间的高纯度基因组DNA
  • 可分离长度不超过50 kb的DNA

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • P2308Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein 3化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书
  • R6148RNase A solution化学品安全说明书

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

法规信息

低风险生物材料
常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000463953
0000463953
0000463905
0000463905
SLCL5155

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Sai Wang et al.
Frontiers in genetics, 11, 585064-585064 (2020-12-01)
Familial renal glucosuria is a rare renal tubular disorder caused by SLC5A2 gene variants. Most of them are exonic variants and have been classified as missense variants. However, there is growing evidence that some of these variants can be detrimental
Yanxia Gao et al.
Renal failure, 36(8), 1226-1232 (2014-07-01)
The objective of this study is to identify ATP6V1B1, ATP6V0A4 and SLC4A1 genes mutations and assess audiologic characteristics in six Chinese children with primary distal renal tubular acidosis from four unrelated families between the ages of 2 and 13 years.
Xiangzhong Zhao et al.
Scientific reports, 6, 33920-33920 (2016-09-27)
Familial renal glycosuria (FRG) is caused by mutations in the SLC5A2 gene, which codes for Na+-glucose co-transporters 2 (SGLT2). The aim of this study was to analyze and identify the mutations in 16 patients from 8 families with FRG. All
Xinsheng Wang et al.
American journal of nephrology, 42(1), 78-84 (2015-09-05)
Twenty-six HOGA1 mutations have been reported in primary hyperoxaluria (PH) type 3 (PH3) patients with c.700 + 5G>T accounting for about 50% of the total alleles. However, PH3 has never been described in Asians. A Chinese child with early-onset nephrolithiasis
Cui Wang et al.
Scientific reports, 6, 33652-33652 (2016-09-21)
Primary hyperoxaluria type 1 (PH1) is a rare genetic disease characterized by excessive oxalate accumulation in plasma and urine, resulting in various phenotypes because of allelic and clinical heterogeneity. This study aimed to detect disease-associated genetic mutations in three PH1
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实验方案

Blood Card - WGA Protocol

Protocol extracts genomic DNA from blood cards, useful for limited DNA samples in amplification.

Extraction & Amplification of Whole Blood Using WGA-Protocol

Simple DNA isolation protocol from whole blood samples for genomic analysis, followed by GenomePlex® amplification.

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

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