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Sigma-Aldrich

GenElute 血液基因组DNA试剂盒

sufficient for 350 purifications

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别名:
血液基因组DNA, Gen Elute

用途

sufficient for 350 purifications

质量水平

technique(s)

DNA purification: suitable

储存温度

15-25°C
room temp

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相关类别

一般描述

GenElute血液基因组DNA试剂盒可以简单、方便地从新鲜或长时间存放(超过24小时)的全血中分离高纯度基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起,同时无需采用昂贵的树脂、乙醇沉淀,以及诸如苯酚和氯仿等有害化学成分。

应用

纯化所得的基因组DNA可用于以下下游应用:
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆

特点和优势

  • 起始材料:不超过200 μl新鲜或长时间存放的血液
  • 预期得率:至多10 μg
  • 洗脱体积:400 μl
  • 所需时间:<40分钟
  • A260/A280比率:1.6 - 1.9
  • 兼容多种抗凝血素、包括EDTA、肝素和柠檬酸钠

原理

起始材料首先溶解在含有离液盐的溶液之中,以确保大分子彻底变性。之后加入乙醇,从而使DNA在溶解物通过微量离心管在硅胶膜上离心时,结合到膜上。随附一份Prewash Solution,可帮助与全血样本长时间存放(超过24小时)相关的污染物。在洗涤去除污染物后,将DNA放在200 mL的Tris-EDTA溶液中洗脱。

预期基因组DNA得率取决于所用的起始材料的数量和性质(例如,从200 μl 新鲜的全血之中,可在一小时内分离出4至10 μg RNase A处理的DNA)。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间,最大长度可达50 kb。

其他说明

更多信息,请见www.sigma-aldrich.com/genomicdna

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • C2112Column Preparation Solution化学品安全说明书

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology化学品安全说明书

  • R6148RNase A solution化学品安全说明书

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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S K Sahu et al.
Oncogenesis, 5, e225-e225 (2016-05-10)
Tumor suppressor p53 is a critical player in the fight against cancer as it controls the cell cycle check point, apoptotic pathways and genomic stability. It is known to be the most frequently mutated gene in a wide variety of
Miri Cohen-Zinder et al.
Physiological genomics, 48(5), 367-376 (2016-03-20)
Ecological and economic concerns drive the need to improve feed utilization by domestic animals. Residual feed intake (RFI) is one of the most acceptable measures for feed efficiency (FE). However, phenotyping RFI-related traits is complex and expensive and requires special
Disha Bhange et al.
Frontiers in microbiology, 12, 779472-779472 (2021-12-14)
In a multicentric, observational, investigator-blinded, and longitudinal clinical study of 764 ART-naïve subjects, we identified nine different promoter variant strains of HIV-1 subtype C (HIV-1C) emerging in the Indian population, with some of these variants being reported for the first
C Castellini et al.
Theriogenology, 78(4), 715-723 (2012-06-19)
There is increasing evidence that the particulate fraction of seminal plasma plays an important role in reproduction of several mammalian species. However, the origin and role of these granules in the physiology of rabbit spermatozoa is partially unknown. The aim
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Cyclooxygenase (COX) enzyme catalyzes the production of prostaglandins, which are important mediators of tissue destruction in periodontitis. Single nucleotide polymorphisms of COX2 enzyme have been associated with increasing susceptibility to inflammatory diseases. The present study evaluates the association of two

实验方案

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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