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安全信息

P1415

Sigma-Aldrich

预杂交溶液

for Northern and Southern blotting, liquid (2x concentrate)

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NACRES:
NA.25

质量水平

形式

liquid (2x concentrate)

异质活性

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

储存温度

−20°C

一般描述

Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液,适用于Northern和Southern印迹方案。

应用

适合用作Northern和Southern印迹方案的预杂交缓冲液。

组分

Sigma的2倍预杂交液含200 μg/mL DNA,溶剂为10倍SSC和2倍Denhardt溶液:
  • 200 μg/mL DNA
  • 2mg牛血清白蛋白(BSA)
  • 2mg聚蔗糖
  • 2mg聚乙烯吡咯烷酮(PVP)
  • 0.15 M柠檬酸钠
  • 1.5M NaCl

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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示例

T1503
货号
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25G
包装规格/数量

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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
T M Lau et al.
Human reproduction (Oxford, England), 11(12), 2629-2634 (1996-12-01)
The expression of endometrial progesterone receptor mRNA during the human menstrual cycle and in Norplant users was studied using digoxigenin-labelled ribonucleic probes for in-situ hybridization on 6 microns paraffin embedded endometrial sections. The staining intensity was scored blind semi-quantitatively. Blood
Elizabeth S Colombo et al.
Investigative ophthalmology & visual science, 48(4), 1793-1800 (2007-03-29)
The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in
Andrew M Pilling et al.
Toxicologic pathology, 35(3), 348-355 (2007-04-25)
Spontaneous follicular and C-cell tumors of the thyroid gland in the Han Wistar rat were examined using two morphologic procedures. Firstly, in situ hybridization (ISH) was used to localize thyroglobulin (TG) and calcitonin (CT) mRNAs. Secondly, the proteins for these
Jianmin Su et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(39), 7421-7435 (2020-08-28)
GABAergic interneurons represent a heterogenous group of cell types in neocortex that can be clustered based on developmental origin, morphology, physiology, and connectivity. Two abundant populations of cortical GABAergic interneurons include the low-threshold, somatostatin (SST)-expressing cells and the fast-spiking, parvalbumin

实验方案

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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