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P4417

Phosphate buffered saline

Name: Phosphate buffered saline
Brand: SIGMA

tablet, pH 7.2-7.6 (1 tablet/200 mL)

别名:

PBS

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关于此项目

UNSPSC Code:

12161700

eCl@ss:

32129211

NACRES:

NA.25

MDL number:

MFCD00131855

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产品名称

Phosphate buffered saline, tablet

form

tablet

Quality Level

200

storage condition

dry at room temperature

color

white

pH

7.2-7.6 (1 tablet/200 mL), 7.4

solubility

water: 1 tablet/200 mL, water: soluble

density

1.02 g/cm³ at 20—25 °C, 1.03 g/cm³ at 20—25 °C

application(s)

advanced drug delivery
diagnostic assay manufacturing
histology
life science and biopharma

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General description

磷酸盐缓冲液（PBS）是一种在生物学研究中得到广泛应用的缓冲溶液，其多功能性和关键属性得到高度认可。特别值得一提的是，其等渗性与人体的pH、渗透压和离子浓度密切相关，它对细胞没有毒性。这种关键特性在多种细胞生物学实验中起到关键作用，包括细胞的温和清洗、组织的安全转运以及细胞和试剂的精确稀释。这些应用有助于保持细胞活力和构建有利的生理环境。PBS的缓冲能力来自于磷酸盐-磷酸的平衡，为实现最佳酶活性和各种生物学过程创建稳定的pH 环境。这种特性令PBS成为试剂制备、蛋白质透析和免疫组织化学等研究工作的理想选择,确保了实验结果的再现性和可靠性.

除了细胞生物学领域外，PBS的多功能性还体现在各种应用中，如为实现最佳抗体-抗原相互作用而构建定制化ELISA缓冲液。此外，它可用作蛋白A/G色谱的高效缓冲液，有助于基于特定抗体结合纯化抗体。在血液学和生物化学操作过程中，PBS 在细胞和组织的有效清洗中起关键作用。总之，PBS是生物学和生物化学研究中不可缺少的工具，具有等渗性、缓冲能力、简单和稳定的特性。其在细胞生物学、试剂制备、蛋白质纯化和各种生物分析中的多样化应用体现了其在增进科学知识和理解中的重要性。

Application

磷酸缓冲盐已用于-

生产铂纳米颗粒(NP-Pt)水解胶体
在SEM之前清洗血液样品和SEM（扫描电子显微镜）存根
制备荧光标记小麦胚芽凝集素(WGA)溶液
在ELISA（酶联免疫吸附测定）期间稀释捕获抗体
在离心过程中悬浮细胞沉淀
免疫测定程序

Features and Benefits

适用于各种研究应用的优质产品
可用作生物学和生物化学应用的缓冲液成分
易溶于水

Preparation Note

将一片溶于200ml去离子水中，在25 °C下得到0.01m磷酸盐缓冲液、0.0027m氯化钾和0.137m氯化钠，pH值为7.4。

Other Notes

如需了解生化试剂系列的更多信息，请填写此表。

存储类别

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Impact of the Conformational Variability of Oligopeptides on the Computational Prediction of Their CD Spectra.

M Michaelis et al.

The journal of physical chemistry. B, 123(31), 6694-6704 (2019-07-11)

Although successful in the structural determination of ordered biomolecules, the spectroscopic investigation of oligopeptides in solution is hindered by their complex and rapidly changing conformational ensemble. The measured circular dichroism (CD) spectrum of an oligopeptide is an ensemble average over

Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins.

Lopez C et al

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Structural damage of chicken red blood cells exposed to platinum nanoparticles and cisplatin.

Kutwin M et al

Nanoscale Research Letters, 9(1), 257-257 (2014)

Alzheimer's Aβ peptides with disease-associated N-terminal modifications: influence of isomerisation, truncation and mutation on Cu2+ coordination.

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PloS one, 5(12), e15875-e15875 (2011-01-07)

The amyloid-β (Aβ) peptide is the primary component of the extracellular senile plaques characteristic of Alzheimer's disease (AD). The metals hypothesis implicates redox-active copper ions in the pathogenesis of AD and the Cu(2+) coordination of various Aβ peptides has been

The adsorption of α-amylase on barley proteins affects the in vitro digestion of starch in barley flour.

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The conversion of barley starch to sugars is a complex enzymic process. Most previous work concerned the biotechnical aspect of in situ barley enzymes. However, the interactions among the macromolecular substrates and their effects on enzymic catalysis has been little

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High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.

实验方案

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Antibody Purification using Protein A/G/L Agarose

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

Immunoprecipitation Procedure

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

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全球贸易项目编号

货号	GTIN
P4417-50TAB	04061835522446
P4417-100TAB	04061835547586

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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