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R0278

Sigma-Aldrich

总蛋白提取细胞裂解缓冲液

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别名:
Radioimmunoprecipitation assay buffer
MDL编号:
MFCD02100484
NACRES:
NA.56

描述

protease free

质量水平

200

形式

liquid

包装

pkg of 50 mL
pkg of 500 mL

技术

immunoprecipitation (IP): suitable

颜色

colorless

pH值（酸碱度）

7.8-8.2 (25 °C, 1 ×)

储存温度

2-8°C

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相关类别

一般描述

RIPA（放射免疫沉淀测定）缓冲液是一种无需准备即可使用的即用型溶液。其可最大限度避免非特异性蛋白质结合相互作用，可维持较低的背景，同时支持实现最具特异性的相互作用，从而可以研究相关蛋白质互作。由于大多数抗体和蛋白质抗原不受该缓冲液成分影响，这种成分广泛用于免疫沉淀等应用。
放射免疫沉淀检测缓冲液（RIPA缓冲液）是一种裂解缓冲液，用于从贴壁和悬浮培养的哺乳动物细胞中快速、有效地进行细胞裂解和蛋白质溶解。

应用

RIPA缓冲液已被用作以下实验研究中的细胞裂解试剂：
  • 基于成纤维细胞的外周组织深度表型分型，有助于对零星帕金森病(sPD)进行机制性疾病分层。
  • 验证氧化磷酸化 (OXPHOS)的抑制作用，这种成分可激活人肺上皮细胞的混合谱系激酶域样蛋白(MLKL)依赖性坏死。

RIPA缓冲液能够有效地进行细胞裂解和蛋白质溶解，并同时避免蛋白质的降解以及对蛋白质的免疫反应性和生物活性干扰。 RIPA缓冲液还可在免疫沉淀和分子沉淀检测中造成低的背景。 与EZview™亲和凝胶兼容。

特点和优势

1 mL RIPA缓冲液可在100 mm培养皿（0.5至5 * 107个细胞）中裂解大多数粘附的哺乳动物细胞系细胞。
将非特异性的蛋白结合互作降低至最低以确保低的背景。

其他说明

含有150 mM NaCl、1.0% IGEPAL® CA-630、0.5%脱氧胆酸钠、0.1% SDS、50 mM Tris, pH 8.0的即用型溶液。

法律信息

EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Solvay

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. After preparing a lysate using Product R0278, RIPA Buffer, how should it be stored? And for how long can the lysate be stored?

    Lysates containing the appropriate inhibitor(s) can be stored at -70°C for up to a year.

  6. Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?

    R0278 can be used on tissue, if it is well homogenized.

  7. Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?

    This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.

  8. How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?

    RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins.  The exact formulation of C2978, however, is proprietary.  

  9. I left the bottle of Product R0278, RIPA buffer, on my bench for about a week. Can I still use it?

    Yes, you should be able to use this product.  Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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