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一般描述
组织样品在TRI reagent®中均质或裂解，即可溶解RNA、DNA和蛋白质。加入氯仿或1-溴-3-氯丙烷，然后离心，混合物将分离为三层：含RNA的水相、含DNA的中间相和含蛋白质的有机相。随后即可通过分离不同相层，得到相应组分。
应用
- 从各种组织样品和细胞中分离总RNA
- 裂解单核细胞
- 从感染SARS-CoV的Vero细胞中分离细胞质RNA
- 从感染甲型流感(H5N1)病毒的MDM（单核细胞衍生的巨噬细胞）中分离总RNA
- 制备SARS-CoV培养物的病毒裂解液，以便为SARS-CoV疫苗开发创建编码S糖蛋白的克隆
使用TRI reagent®分离的RNA、DNA和蛋白质可继续用于各种下游应用，如克隆、PCR、RT-PCR、Northern印迹、mRNA分离、体外翻译、RNase保护测定、限制酶消化、Southern印迹、SDS-PAGE和蛋白质印迹。
特点和优势
多用途：TRI Reagent®同时适于大量或少量组织或细胞，同时支持多种类型的样品，包括人、植物、酵母、细菌和病毒来源样品。
高效：相对于传统硫氰酸胍/氯化铯方法，TRI reagent®产量更高。从新鲜组织或细胞开始的完整RNA提取流程可在一小时内完成。
- 可轻松扩展的RNA分离
- 可适用于多种来源：人、植物、酵母、细菌或病毒
- 相对于传统的硫氰酸胍/氯化铯具有更高的产量
法律信息
相关产品
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
补充剂危害
储存分类代码
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 2
闪点(°F)
174.2 °F - closed cup
闪点(°C)
79 °C - closed cup
法规信息
分析证书(COA)
输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。
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如何查找产品货号
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示例
其它示例：
705578-5MG-PW
PL860-CGA/SHF-1EA
MMYOMAG-74K-13
1000309185
输入内容 1.000309185)
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如何查找COA批号
批号可以在产品标签上"批“ （Lot或Batch）字后面找到。
Aldrich 产品
如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。
未找到您寻找的产品？
部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。
My cells are plated on polystyrene culture plates. How do I use Product T9424, TRI Reagent in this application?
We recommend harvesting the cells in PBS with a rubber policeman/cell scraper, transfer to an eppendorf tube, and proceed with the RNA isolation by removing the PBS from the cell pellet and re-suspending the pellet in the Tri Reagent.
If I add a volume of Product T9424, Tri Reagent™, different from 1 ml, how much chloroform and isopropanol should I add?
What is important is to keep the ratio the same. For example, we recommend adding 200 μL of chloroform and 500 μL of isopropanol when using 1 ml of Tri Reagent. Therefore, 100 μL of chloroform and 250 μL of isopropanol would be added if using 500 μL of Tri Reagent. Similarly, 300 μL of chloroform and 750 μL of isopropanol would be added to if using 1.5 ml of Tri Reagent.
What is the expiration date for Product T9424, Tri Reagent™?
The expiration date for T9424 has not been established. However, based on our storage guidelines, it should be usable for 1 year from the date of receipt.
Can I use T9424 Tri Reagent™ for blood samples?
No, we recommend using T3809 Tri Reagent BD for work in blood samples.
Which chloroform and isopropanol do you recommend to be used with T9424, Tri Reagent™?
For chloroform and isopropanol we recommend C2432 and I9616, respectively.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
实验方案
TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
TRI试剂是一种单步RNA分离试剂，可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法，以及各种样品提取方法和故障排除方案。
使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程
相关内容
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
细胞裂解和蛋白质溶解是进行有效分析和高效处理的关键步骤，默克分享提取方法包括酶法、化学法、机械法或几种方法的联合使用。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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