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文件

B9285

Sigma-Aldrich

5-溴-2′-脱氧尿苷

BioUltra, ≥99%

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别名:
5-BrdU, 5-溴-1-(2-脱氧-β-D-呋喃核糖)尿嘧啶, 5-溴脱氧尿苷, BUdR
经验公式（希尔记法）:
C9H11BrN2O5
CAS号:
59-14-3
分子量:
307.10
Beilstein:
30395
EC 号:
200-415-9
MDL编号:
MFCD00006529
PubChem化学物质编号:
24892132
NACRES:
NA.51

生物来源

synthetic (organic)

产品线

BioUltra

检测方案

≥99%

形式

powder

杂质

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

灼烧残渣

≤0.1%

mp

191-194 °C (dec.) (lit.)

溶解性

NH4OH: 0.1 M at 20 °C, clear, colorless

痕量阳离子

Al: ≤0.0005%
Ca: ≤0.01%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

储存温度

−20°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

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相关类别

一般描述

5′-溴-2-脱氧尿苷(BrdU)是一种可被皮肤吸收或吸入的致癌物质。BrdU溶液对光敏感。

应用

5-溴-2′-脱氧尿苷已用于：
  • 在细胞增殖测定中标记妊娠小鼠的增殖细胞
  • 研究缺血后不同时间的细胞增殖时程确认MKI67 +细胞在细胞周期中重新进入生殖细胞
  • 成纤维细胞5′-溴-2′脱氧尿苷(BrdUrd)染色

生化/生理作用

5′-溴-2-脱氧尿苷(BrdU)染色主要用于体内观察肿瘤细胞和其他组织的增殖。
5-溴-2′-脱氧尿苷（5-BrdU）是一种胸苷类似物，可掺入DNA中。 5-BrdU通常广泛用于测定DNA合成和标记分裂细胞。 因此，5-BrdU常用于研究细胞信号传导和诱导细胞增殖的其他过程。
用作遗传研究中诱变剂的胸苷类似物。在 S 期可选择性结合到细胞 DNA 中。

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H340 - H361fd

危险分类

Muta. 1B - Repr. 2

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

个人防护装备

Eyeshields, Gloves, type N95 (US)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product B9285, 5-Bromo-2´-deoxyuridine, be injected into animals?

    Sigma has not injected animals with solutions of this compound, so we do not have a stepwise procedure for you.  However, this application has been reported in the literature.  Please see the cited references below, which you may find useful. Several references cite the use of Product No. B9285 in their protocols.1. Yin, X-X, et al., Expression of active caspase-3 in mitotic and postmitotic cells of the rat forebrain. J. Comp. Neurol., 433(1), 4-22 (2001).  Injected into mice at 50 mg/kg.2. Dackor, R.T., et al., Hydrops Fetalis, Cardiovascular Defects, and Embryonic Lethality in Mice Lacking the Calcitonin Receptor-Like Receptor Gene. Mol. Cell. Biol., 26(7), 2511-2518 (2006).  Injected into mice at 100 mg/kg.3. Farris, S.M., et al., Larval and Pupal Development of the Mushroom Bodies in the Honey Bee, Apis mellifera. J. Comp. Neurol., 414, 97-113 (1999).  A 25 mg/mL solution was injected into bee larvae, prepupae, and pupae.4. Gaiano, N., et al., Radial Glial Identity Is Promoted by Notch1 Signaling in the Murine Forebrain. Neuron, 26(2), 395-404 (2000).  A 20 mg/mL solution was injected into mice.

  6. For how long is Product B9285, 5-Bromo-2´-deoxyuridine, stable in solution?

    5-Bromo-2´-deoxyuridine is stable in solution for 6 months when stored -20°C.

  7. In what solvents is Product B9285, 5-Bromo-2´-deoxyuridine, soluble?

    Solubility information for 5-bromo-2´-deoxyuridine can be found on the product information sheet (under Documents, above).

  8. What is the shelf life of Product No. B9285 (5-bromo-2´-deoxyuridine)?

    Sigma-Aldrich does not assign an expiration date to this product; it is guaranteed for one year from the date of shipment.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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