Merck
CN

M3671

Sigma-Aldrich

MES

low moisture content, ≥99% (titration)

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别名:
2-(N-吗啉基)乙磺酸, 4-吗啉乙磺酸
经验公式(希尔记法):
C6H13NO4S
CAS号:
分子量:
195.24
Beilstein:
141319
EC 号:
MDL编号:
eCl@ss:
32129211
PubChem化学物质编号:
NACRES:
NA.25

质量水平

检测方案

≥99% (titration)

形式

powder

杂质

≤1.0% water

pH值(酸碱度)

2.5-4 (25 °C, 97.6 g/L)

useful pH range

5.5-6.7

pKa 

6.1

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma (
)
metabolomics (
)

SMILES string

OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)

InChI key

SXGZJKUKBWWHRA-UHFFFAOYSA-N

相关类别

一般描述

MES缓冲液是专为生物学和生化应用开发的Good′s缓冲液之一,在各种研究中发挥重要作用。这种两性离子吗啉缓冲液特别有价值的一点是它在5.5~6.7的pH范围内具有缓冲能力。尤其是MES缓冲液不会与环境和生物学研究所用的大多数金属形成络合物。它易溶于水却极难溶于脂质,因而无法透过膜。

这种缓冲液应用广泛,可用于在细胞培养基、基于蛋白的缓冲配方和电泳缓冲液中维持环境稳定。其重要性也见于抗体、多肽、蛋白质、血液成分和生长因子的纯化过程。MES缓冲液也是毛细管电色谱的良好选择,具有很低的离子迁移率。重要的是,它可作为二甲胂酸盐和非两性离子缓冲液(如柠檬酸盐和苹果酸盐)等有毒试剂的更安全的替代品。在各种实验环境中,MES缓冲液也用于精细调节生长培养基的pH值,稳定酶溶液,并可作为PAGE电泳缓冲液的成分。

应用

MES已被用作洗脱缓冲液组分,可从α2-巨球蛋白亲和柱上洗脱血浆。在抗体与微粒结合过程中,这种成分用作活化缓冲液。在创建创建用于病原体检测的微流集成表面等离振子共振(SPR)平台的过程中,MES可用于微通道官能化。
可用作培养基的碳酸氢盐缓冲液‐

特点和优势

  • 经检测确认重金属污染水平低,适合各种应用
  • 有效缓冲范围pH 5.5-6.7 (25 °C),pKa 6.1 (25 °C)
  • 极易溶于水
  • 金属离子结合性极弱
  • 细胞毒性低于Tris和磷酸盐等缓冲液
  • 大范围pH值内保持稳定
  • 紫外吸收低,反应性极弱

质量

低水分

其他说明

For additional information on our range of Biochemicals, please complete this form.

可比产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

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示例

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货号
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705578-5MG-PW

PL860-CGA/SHF-1EA

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1000309185

输入内容 1.000309185)

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What does low moisture content mean in the description of Product M3671, MES - low moisture?

    The amount of water/moisture for MES (M3671) is tested by Karl Fisher and meets a specification of less than 1%. The other MES Hydrates offered have a specification of less than 9%.

  4. What is the solubility of Product M3671, MES - low moisture?

    We test the solubility of Prod. No. M3671 at 20 grams in 80 mL of water. A clear, colorless solution is obtained. This concentration is not necessarily the upper solubility limit of MES in water.

  5. What is the pH of a solution of Product M3671, MES - low moisture?

    The pH of a 0.5 M solution in water should be between 2.5 and 4.0.

  6. Is Product M3671, MES - low moisture, autoclavable?

    Autoclaving is not recommended for any sulfonic acid buffer solution. Sterilization of a MES buffer solution can be accomplished by filtering it through a 0.2 micron filter.

  7. How does one prepare a buffer using Product M3671, MES - low moisture, free acid?

    A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH. Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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J. Virol. Methods, 178, 31-38 (2011)
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Tokel, Onur, et al.
Scientific Reports, 2015, 9152-9152 null
Impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-? is an early biomarker for mild cognitive impairment preceding Alzheimer's disease.
Sagare, Abhay P., et al.
Journal of Alzheimer'S Disease, 24 (1), 25-34 (2011)
Sensitive Mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (PRRSV) from lung tissue samples in a microfluidic chip.
Song JY, Lee CH, Choi EJ, Kim K, et al.
J. Virol. Methods, 178, 31-38 (2011)
"(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review.
Ferreira, Carlos MH, et al.
Royal Society of Chemistry Advances, 5 (39), 30989-31003 (2015)

实验方案

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

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