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Merck
CN

N3254

4-硝基苯磷酸盐 二(三羟甲基氨基甲烷) 盐

phosphatase substrate, ≥97% (enzymatic), powder

别名:

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐

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关于此项目

线性分子式:
(C6 H4 N O6 P)( C4 H12 N O3)2
化学文摘社编号:
68189-42-4
分子量:
461.36
NACRES:
NA.83
PubChem Substance ID:
24897633
UNSPSC Code:
12352204
EC Number:
269-198-6
MDL number:
MFCD00037009
Beilstein/REAXYS Number:
1578142

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产品名称

4-硝基苯磷酸盐 二(三羟甲基氨基甲烷) 盐, phosphatase substrate

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

assay

≥97% (enzymatic)

form

powder

solubility

water: 50 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

Quality Level

100

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相关类别

Application

4-硝基苯磷酸盐双(三羟甲基氨基甲烷)可用于：
  • 作为底物定量分析细胞裂解液中的抗酒石酸酸性磷酸酶(TRAP)活性
  • 天然聚丙烯酰胺凝胶电泳(PAGE)测定磷酸酶活性
  • 在酶联免疫吸附测定(ELISA)中用于碱性磷酸酶

Biochem/physiol Actions

对硝基苯磷酸(pNPP)具有高灵敏性，在酶联免疫吸附测定(ELISA)中特别适合作为碱性磷酸酶作用底物。该底物反应生成可溶性黄色终产物，可在405nm处通过分光光度法读取。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN1064
BCCJ4964
BCCG8128
BCBX4816
BCBS1398V

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B Le Gars Santoni et al.
Bioactive materials, 10, 222-235 (2021-12-14)
β-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on
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商品

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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Product Information Sheet - N3254

Product Information Sheet

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