MISSION^® miRNA Product Offerings

• Peptide transfection reagent for use in difficult to transfect cells

Choose from over 2500+ miRNA mimics

• Most comprehensive collection of miRNA human mimics available (mirBase ver. 21)
• Available as a complete library or individual miRNA mimics
• Stable, long-term expression of miRNA
• Efficient delivery of mimic into wide variety of cell types
• The ability to select for cells that express the miRNA of interest
• Custom options available

• Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content.

Most comprehensible list of miRNA mimics

• Designed using a proprietary algorithm based upon the Tough Decoy (TuD) design
• Long-lasting inhibition at very low nM concentrations
• Excellent resistance to cellular nucleases
• Structure provides potent inhibition of the desired miRNA
• Custom synthesis capabilities: custom species sequences, quantities, and modifications

Choose from over 3,000+ human and mouse miRNA inhibitors

• Designed using a proprietary algorithm based upon the Tough Decoy (TuD) design
• Allows for potent inhibition of the desired miRNA
• Stable, long-term expression of miRNA
• Efficient delivery of mimic into wide variety of cell types
• Species include both human and mouse: 1,953 human lenti miRNA inhibitors and 1,214 mouse lenti miRNA inhibitors
• Custom options available

Different gene pathway of interest? Want a more complete list?

Bench-top transcriptome-wide human miRNA and ncRNA gene target identification.

• Convenience: Perform dual screening with one vector due to the innovative dual selection cassette
• Simplicity: 3 easy steps: Library Transfection, microRNA Transfection and Ganciclovir Selection, and PCR and Sequencing.
• Relevance: The only method for genome-wide functional identification of miRNA targets
• Custom miRNA mimic synthesis capabilities: custom species sequences, quantities, and modifications

Validate miRNA targets and study post-transcriptional gene regulation with lentiviral 3′UTR reporters.

• A genome-wide collection of pre-cloned reporter constructs for over 10,000 human 3’UTRs
• Constructs are composed of a human 3’UTR fused to a novel luciferase reporter
• Relevance: The only method for genome-wide functional identification of miRNA targets
• Optimized Renilla luciferase delivers higher activity
• System utilizes a lentiviral vector for delivery into difficult to transfect cell lines, including differentiated and non-dividing cells
• Superb virus quality delivers superior performance
• Negative controls, miRNA Mimics, and MISSION LightSwitch Luciferase Assay Reagent^™ are available to complete the validation workflow.

Choose from over 2500+ miRNA mimics

• Most comprehensive collection of miRNA human mimics available (mirBase ver. 21)
• Available as a complete library or individual miRNA mimics
• Stable, long-term expression of miRNA
• Efficient delivery of mimic into wide variety of cell types
• The ability to select for cells that express the miRNA of interest
• Custom options available

Lentiviral and Synthetic miRNA Negative Controls Measure transduction efficiency or monitor off-target effects.

• All miRNA mimic and inhibitor negative controls are carefully selected, and have no known homology to known human or mouse gene sequences
• Each negative control contains either a Arabidopsis thaliana sequence (Control 1) or a Caenorhabditis elegans sequence (Control 2)
• Lenti TuD Negative controls available: HLTUD001C and HLTUD002C
• Lenti miR Negative Controls available: NCLMIR001 and NCLMIR002
• Synthetic miRNA mimics Negative Controls available as HMC0002 and HMC0003
• Synthetic miRNA Inhibitors Negative Controls available as NCSTUD001 and NCSTUD002