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106-70-7
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关键词:'106-70-7'
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The Effective Use of Protein Kinase Inhibitors
96 98 89
PKA 2 91 35 17 94 95 105 106 96 66 97 91 104 104 96 96 70 99 87
PKCα 79 98 86 95 95 92 99 93 89 92 100 91 70 99
The Effective Use of Protein Kinase Inhibitors
96 98 89
PKA 2 91 35 17 94 95 105 106 96 66 97 91 104 104 96 96 70 99 87
PKCα 79 98 86 95 95 92 99 93 89 92 100 91 70 99
TB314 TriEx™ Sf9 Cells
28°C medium
to final concentration of 0.5 × 106 cells/ml. Transfer to suspension culture.
Trypan Blue exclusion method
1. Add 200 µl cells (from Step 7 of Thawing TriEx™ Sf9 Cells) to 200 µl Trypan
User_Guide-HLP400
100,000 5,600 2.15 x 106
95 0.32 100,000 32,000 3.07 x 106
48 1.02 100,000 102,000 4.89 x 106
24 1.94 100,000 194,000 4.66 x 106
12 3.87 100,000 387,000 4.64 x
TB329 Sf9 Insect Cells
Flask Size Cell Number Medium Volume
25-cm2 flask 1.0 × 106 5 ml
75-cm2 flask 3.0 × 106 10 ml
150-cm2 flask 6.0 × 106 30 ml
Passaging Suspension Cultures
Sf9 Insect
Product Information Sheet - VP002
P20
P21
P22
P23
P24
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Data Sheet - 102376
Passage when VCD is
above 1.5 × 106 cells/mL
• When the culture is stable with a viability of > 95%
and a cell density above 1.5 × 106 cells/mL
• Freeze some cells (WCB 7%, with 1
Product Information Sheet - P5380
°C), for 10 minutes (activity
remaining):19
• pH 6 ≈ 70%
• pH 7 ≈ 80%
• pH 7.5-10 ≈ 95%
• pH 10.5 ≈ 90%
• pH
Cell Culture Media 4CHO Sample Kit
cells/mL) 0.5 x 106
End of assay Day 14 or if cell viability decreases to 70%
Determine cell density and viability At day of feeding and day 14
Determine product titer at day 7, 10, 12, 14
Mobius®CellReady 50 L and 200 L Single-use Bioreactor Systems Performance Guide
CellReady 3 L satellites
Experiment Volume
[L]
Growth Rate
[1/hr]
Peak VCD
[106 cells /mL]
Day 7
[% viable]
Peak Lactate
[g/L]
Experiment 1 50 0.031 27 97 2.2
2 0.033 23
User Guide: Sf-RVN® Platform
100 x 106 cells required
If the cell density is 4.5 x 106 viable cells/mL:
7. Transfer the calculated volume of cell suspension to
one or more 15, 50, or 200 mL conical centrifuge
tubes.
8. Centrifuge
CDK1/CyclinA2 active (C0244)
,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼79 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 78–106 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
Process Guidance Cellvento™ BHK-200 Medium
the viable cell density
reaches 1.5×106 cells/mL
• Before proceeding to the next step (reduced serum concentration), a minimal viable cell density of
1.5×106 cells/mL and a viability > 95% should
A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
Condition Cell Number per 6-Well Dish
HEScGRO Accumax 1.7 x 106
KOSR Collagenase 1.3 x 106
HEScGRO Manual 0.5 x 106
KOSR Manual 1.6 x 106
0.000
0.200
0.400
0.600
Product Information Sheet-11835246001
12Apoptotic DNA-Ladder Kit
sigma-aldrich.com 7
2.2. Protocols
Before starting a purification reaction, warm the Elution buffer (Bottle 3) to +70°C; all other reagents
should be at +15 to +25°C.
Innovative technology to stabilize DNA at room temperature in tissues and cells
293T cells2 2.8 µg 2.6 µg
Sequencing of rat β-actin from kidney samples stored 70 days in
DNAgard
Figure 7 Genomic DNA in tissue specimens is stabilized by DNAgard as
demonstrated by sequencing
ChemiScreen™ FLASH AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
-8 -7 -6 -5 -4
-10
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[Carbachol] Log M
∆∆ ∆∆
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-10 -9 -8
TB009 Competent Cells
transformation efficiency 2 × 106 cfu/µg Test Plasmid
0.4 ml
1 ml
69041-3
69041-4
B834(DE3)pLysS Competent Cells
guaranteed transformation efficiency 2 × 106 cfu/µg Test Plasmid
0.4 ml
Amide-protected building blocks for smart peptide synthesis
prion peptide (PrP) 106-126 [5] was
performed using standard derivatives and with Dmb-dipeptides
introduced at the position indicated in Figure 7.
Fig. 7: Primary sequence of PrP (106-126). The residues
Product Information Sheet
resulting lysate may be stored at –70 °C
for several months.
• Pelleted cells: Vortex pellet to loosen cells. Add 250 µL of
the Lysis Solution/2-ME Mixture for up to 5 × 106 cells or
500 µL of the
Technical Bulletin - T9424
; placenta, 1-4 g.
c. Typical yields from cultured cells
(g RNA/106 cells): epithelial cells, 8-15 g;
fibroblasts, 5-7 g.
d. Ethidium bromide staining of RNA in agarose
gels visualizes
Anti-Connexin-32 (106-124) antibody produced in rabbit (C3595)
Anti-Connexin-32 (106-124) antibody produced in rabbit (C3595) - Datasheet
ANTI-CONNEXIN 32 (106-124)
Developed in Rabbit, Affinity Isolated Antibody
Product Number C3595
Product
Product Information Cellvento™ BHK-200 Medium
Passage when VCD is above 1.5 × 106 cells/mL
• When the culture is stable with a viability of > 95% and a cell density
above 1.5 × 106 cells/mL
• Freeze some cells (WCB 7%, with 1
TB214VM pSTBlue-1 Vector
3510)
Sap I(3627)
T7 start(41)
Kpn I(55)
Sph I(63)
Pst I(68)
Mlu I(70)
SnaB I(78)
BamH I(84)
EcoR I(91)
EcoR V(101)
EcoR I(106)
Sal I(112)
Acc I(113)
Hinc II(114)
Hind III(118)
Xho I(124)
Avr II(
ChemiScreenTM GLOW AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
9 -8 -7 -6 -5 -4
0
10
20
30
40
50
60
70
80
90
100
110
120
[Carbachol] Log M
∆∆ ∆∆
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-10 -9
Product Information Sheet: Cellvento® BHK-200 cell culture medium
Passage when VCD is
above 1.5 × 106 cells/mL
• When the culture is stable with a viability of > 95%
and a cell density above 1.5 × 106 cells/mL
• Freeze some cells (WCB 7%, with 1
Microsoft Word - VP001 tech bulletin final
bioreactor at 1 106 viable cells/mL (vc/mL) and in the 3 L bioreactor at
5 105 vc/mL, comparable growth of cells was achieved at both scales. Peak
viable cell density (VCD) of 16–20 106 vc/mL was obtained
Data Sheet - L1792
ships on dry ice and storage at −70 °C is
recommended. The product, as supplied, remains
active for at least 2 years.
References
1. DeWet, J.R., et al., Mol. Cell. Biol., 7, 725-737
(1987).
2. Stanley
TB459 BacMagic™ DNA Kits
"10-7", and "control". Add Sf9 cells (0.9 x 106 cells/plate).
Leave plates at room temperature for 1 h on a level surface.
2. During incubation period, label 7 sterile tubes from 10-1 to 10-7 for
Process Guidance - Cellvento BHK-200
the
viable cell density reaches 1.5×106 cells/mL
• Before proceeding to the next step (reduced serum concentration), a minimal viable cell
density of 1.5×106 cells/mL and a viability > 95% should
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