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Stephen P Miller et al.
Chembiochem : a European journal of chemical biology, 15(8), 1145-1153 (2014-05-07)
An active site lysine essential to catalysis in isocitrate dehydrogenase (IDH) is absent from related enzymes. As all family members catalyze the same oxidative β-decarboxylation at the (2R)-malate core common to their substrates, it seems odd that an amino acid
V M Bondarenko et al.
Zhurnal mikrobiologii, epidemiologii, i immunobiologii, (5)(5), 66-70 (2010-01-13)
To assess sorption properties of Spherocelle beads consisting of particles of macroporous celiulose with various charges in relation to bacterial cells of manufacturing probiotic strains from different taxonomic groups. The following manufacturing strains: Bifidobacterium bifidum 1, Lactobacillus plantarum 8PA-3 and
H P Lesch et al.
Gene therapy, 18(6), 531-538 (2011-01-21)
Lentivirus can be engineered to be a highly potent vector for gene therapy applications. However, generation of clinical grade vectors in enough quantities for therapeutic use is still troublesome and limits the preclinical and clinical experiments. As a first step
T Tharmalingam-Jaikaran et al.
Reproduction (Cambridge, England), 148(6), 569-580 (2014-09-13)
Follicular fluid (FF), an important microenvironment for the development of oocytes, contains many proteins that are glycosylated with N-linked glycans. This study aimed i) to present an initial analysis of the N-linked glycan profile of bovine FF using hydrophilic interaction
Pan Yi et al.
Carbohydrate polymers, 121, 183-189 (2015-02-11)
In this work, crude polysaccharide extracts were obtained from mycelia of the edible fungus Ganoderma capense (Lloyd) Teng. After removal of proteins by the Sevage method, fractionation and purification by anion-exchange and gel-permeation chromatography, a polysaccharide (GCPB-3) was isolated. The
CO2 absorption/desorption in aqueous DEEA/MDEA and their hybrid solutions with sulfolane
Nozaeim, et al.
Journal of Natural Gas Science and Engineering, 76, 103219-103219 (2020)
Glucamines as green alternatives to conventional amino alcohols in metalworking fluids
Seddig, et al.
Materials and Corrosion, 74, 394-402 (2023)
Elizabeth C Schramm et al.
Blood, 125(15), 2359-2369 (2015-01-23)
The pathogenesis of atypical hemolytic uremic syndrome (aHUS) is strongly linked to dysregulation of the alternative pathway of the complement system. Mutations in complement genes have been identified in about two-thirds of cases, with 5% to 15% being in C3.
Karl B McCann et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 969, 241-248 (2014-09-10)
The manufacture of human serum albumin by chromatographic procedures involves gel filtration chromatography as a final polishing step. Despite this step being essential to remove high molecular weight impurity proteins and thus ensure a stable and safe final product, it
Marcella Sarzotti-Kelsoe et al.
Journal of immunological methods, 409, 147-160 (2014-03-13)
A3R5 is a human CD4(+) lymphoblastoid cell line that was engineered to express CCR5 and is useful for the detection of weak neutralizing antibody responses against tier 2 strains of HIV-1. Here we describe the optimization and validation of the
Alfred Mugambi Mariga et al.
Journal of the science of food and agriculture, 94(15), 3152-3162 (2014-03-22)
Currently, the use of mushrooms as functional foods, nutraceuticals or phytopharmaceuticals source has risen. In contrast, the possible cellular cytotoxicity and immunostimulatory activity of Pleurotus eryngii (DC. ex Fr.) Quel protein (PEQP) is unknown. Here we report extraction, anti-tumorigenic and
Philip M Cummins et al.
Methods in molecular biology (Clifton, N.J.), 681, 215-228 (2010-10-28)
Ion-exchange chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful resolving ability, and ease of scale-up and automation
Ke Zheng et al.
International journal of nanomedicine, 10, 5327-5342 (2015-09-09)
Doxorubicin (DOX) is an effective chemotherapy drug used to treat different types of cancers. However, DOX has severe side effects, especially life-threatening cardiotoxicity. We herein report a new approach to reduce the toxicity of DOX by embedding DOX inside human
Amino alcohol additives for the fast living radical polymerization of methyl methacrylate with RuCl2 (PPh3)3.
Ando T, et al.
Journal of Polymer Science Part A: Polymer Chemistry, 41(22), 3597-3605 (2003)
James DelProposto et al.
Protein expression and purification, 63(1), 40-49 (2008-10-01)
A persistent problem in heterologous protein production is insolubility of the target protein when expressed to high level in the host cell. A widely employed strategy for overcoming this problem is the use of fusion tags. The best fusion tags
Pamela Y Sandoval et al.
PLoS genetics, 11(7), e1005405-e1005405 (2015-07-29)
The intra-S phase checkpoint kinase of metazoa and yeast, ATR/MEC1, protects chromosomes from DNA damage and replication stress by phosphorylating subunits of the replicative helicase, MCM2-7. Here we describe an unprecedented ATR-dependent pathway in Tetrahymena thermophila in which the essential
Yu Ji et al.
Journal of chromatography. A, 1348, 137-149 (2014-05-23)
Multivalent pneumococcal vaccines were used worldwide to protect human beings from pneumococcal diseases. In order to eliminate the toxic organic solutions used in the traditional vaccine purification process, an alternative chromatographic process for Streptococcus pneumoniae serotype 23F capsular polysaccharide (CPS)
Yun-xiang Zang et al.
Journal of Zhejiang University. Science. B, 16(8), 696-708 (2015-08-05)
Glucosinolates (GSs) are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid (JA) and salicylic acid (SA) are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect
Anders Wittrup et al.
Methods in molecular biology (Clifton, N.J.), 683, 99-115 (2010-11-06)
Cell-penetrating peptides (CPPs) are widely used to deliver macromolecular cargoes to intracellular sites of action. Many CPPs have been demonstrated to rely on cell surface heparan sulfate proteoglycans (HSPGs) for efficient cellular entry and delivery. In this chapter, we describe
V M Bondarenko et al.
Zhurnal mikrobiologii, epidemiologii, i immunobiologii, (6)(6), 3-8 (2012-02-09)
Study of sorption properties of various spherical polysaccharide matrixes designated as Spherocell to probiotic Lactobacillus plantarum 8RA-3 bacteria. Industrial strain of L. plantarum 8PA-3 was used. The process of immobilization of lactobacilli on 3 variants of spherical sorbents was studied.
Serwanja Jamil et al.
Applied biochemistry and biotechnology, 176(5), 1335-1350 (2015-06-05)
Due to its systemic arginine degradation, arginine deiminase (ADI) has attracted attentions as an anti-tumor drug. Its low activity at physiological conditions among other limitations has necessitated its engineering for improved properties. The present study describes the hydrophobic mutagenesis and
Peter H Otto et al.
Veterinary microbiology, 179(3-4), 168-176 (2015-08-01)
Rotaviruses (RVs) are a major cause of neonatal diarrhoea in humans and animals worldwide. In this study, 425 faecal samples were collected between 1999 and 2013 from diarrhoeic livestock and companion animals at different locations in Germany and tested for
Jun-Hui Choi et al.
Biochimie, 105, 45-57 (2014-07-01)
A bi-functional fibrinolytic serine protease, Starase exhibiting thrombolytic potency was purified from hepatic caeca of Asterina pectinifera. Starase showed a single band of approximately 48 kDa by SDS-PAGE and fibrin zymography. The N-terminal sequence of Starase was AIPTEFDARTKKHNN, which does not
Hannah E Rockwell et al.
ASN neuro, 7(2) (2015-04-16)
Sandhoff disease (SD) is an autosomal recessive neurodegenerative disease caused by a mutation in the gene for the β-subunit of β-N-acetylhexosaminidase (Hex), resulting in the inability to catabolize ganglioside GM2 within the lysosomes. SD presents with an accumulation of GM2
Xuejing Jia et al.
Molecules (Basel, Switzerland), 20(11), 20901-20911 (2015-11-28)
Box-Behnken design (BBD), one of the most common response surface methodology (RSM) methods, was used to optimize the experimental conditions for ultrasound-assisted extraction of polysaccharides from Rhynchosia minima root (PRM). The antioxidant abilities and anticancer activity of purified polysaccharide fractions
Haitian Fan et al.
The Journal of biological chemistry, 290(34), 20856-20864 (2015-07-15)
The translational GTPase BipA regulates the expression of virulence and pathogenicity factors in several eubacteria. BipA-dependent expression of virulence factors occurs under starvation conditions, such as encountered during infection of a host. Under these conditions, BipA associates with the small
Debanjan Bhattacharya et al.
Experimental and molecular pathology, 98(3), 455-466 (2015-03-24)
Malignant gliomas represent one of the most aggressive and hypervascular primary brain tumors. Angiopoietin-1, the peptide growth factor activates endothelial Tie-2 receptor promoting vessel maturation and vascular stabilization steps of angiogenesis in glioma. Epidermal growth factor receptor (EGFR) and Tie-2
Guodong Feng et al.
The Analyst, 136(22), 4822-4829 (2011-10-04)
A new ion-exchange adsorbent (IEA) derived from Fe(3)O(4)/SiO(2)-GPTMS-DEAE with paramagnetic properties was prepared. Fe(3)O(4) nanoparticles were firstly prepared in water-in-oil microemulsion. The magnetic Fe(3)O(4) particles were modified in situ by hydrolysis and condensation reactions with tetraethoxysilane (TEOS) to form the
Xiang-Yu Cao et al.
Molecular medicine reports, 12(2), 2383-2389 (2015-04-22)
The present study aimed to determine the antitumor effects of polysaccharides extracted from Pleurotus ostreatus mycelium on gastric cancer in vitro and in vivo. Polysaccharides were extracted from Pleurotus ostreatus mycelium and an antitumor component, known as Pleurotus ostreatus mycelium
Tiarnan D L Keenan et al.
Investigative ophthalmology & visual science, 55(8), 5370-5379 (2014-07-31)
Heparan sulfate (HS) has been implicated in age-related macular degeneration (AMD), since it is the major binding partner for complement factor H (CFH) in human Bruch's membrane (BrM), and CFH has a central role in inhibiting complement activation on extracellular
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