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To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.

Order shRNA Online in 5 Easy Steps

5 easy steps to order shRNA gene of your interest.

5'-核苷酸酶的酶促检测

在测定5′-核苷酸酶活性时，本操作流程使用5′-单磷酸腺苷和一种显色试剂生成的标准曲线而对释放的磷的微摩尔数进行测定。

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

Conjugated Polymers for the Engineering of Device/Tissue Interface

Conjugated polymers offer charge transport between inorganic, electrically conducting metals and organic, proton-conducting biological systems.

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Download our Training Program

Our Training Program is availabale in 5 different languages.

Puriﬁcation using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Hydroxymethylfurfural Analysis of Beer by HPLC UV

HPLC-UV determination of 5-HMF (5-(hydroxymethyl)furfural) in beer samples using monolithic silica Chromolith® HighResolution RP-18e HPLC column.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

细胞培养实验方案5：悬浮细胞系的传代培养

用于传代和分装悬浮细胞系的细胞培养方案

5 Reasons Cancer Researchers Adopt 3D Cell Culture: A Review of Recent Literature

Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology.

默克生命科学官网2周年庆，多重惊喜限时开启！

默克生命科学官网2周年庆！ 2023年4月1日到5月31日，多重惊喜限时开启。

癌症研究人员采用 3D 细胞培养的5个原因：近期文献综述

许多癌症模型系统可用于调查疾病机制和筛选疗法。虽然所有的模型都提供了有关癌症生物学的重要信息。

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Reflectometric Determination of Hydroxymethylfurfural in Honey

Rapid analysis of 5-(Hydroxymethyl)furfural (HMF) in honey using a reflectometer and Reflectoquant® HMF Test.

Duolink In Situ后复染实验方案

我们建议在Duolink In Situ 荧光用户手册的第7.3节第5步完成扩增步骤后进行复染操作。

Critical Inhibitor Characteristics to Consider

Discover critical characteristics to consider when working with enzyme inhibitors, such as cell permeability, the prozone effect, and Lipinski’s rule of 5.

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Block Copolymers in Nanoscale Patterning

Block copolymers coat surfaces for nanoscale patterns, improving resolution to 5-50nm, enabling diverse applications like printable circuits.

细胞培养污染问题排查_哺乳动物细胞培养-默克生命科学

细胞污染有多普遍？根据FDA、ATCC和其他人的研究，估计单被支原体污染的细胞培养物占5-30%。在一项研究中，常见细胞系的病毒污染发生率超过25%5，非致细胞病变病毒甚至比支原体更难检测，因为培养物健康无法反映前者的存在。

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

用于核酸去磷酸化的小牛肠（CIP）碱性磷酸酶

CIP可用于脱去DNA、RNA及核糖和脱氧核糖核苷三磷酸上的5’-磷酸基团。如何进行DNA去磷酸化的详细实验方案。

地高辛 (DIG) 标记方法

地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Integrity Testers

Efficient Filter Integrity Testing: Elevate quality control with Integritest® 5, offering bubble point, diffusion, and hydrophobic testing in-line or offline.

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

QuEChERS Method for Pesticide Residue Analysis

QuEChERS method for pesticide residue analysis requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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