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B0149
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Pablo Cassaglia et al.
The American journal of pathology, 190(9), 1789-1800 (2020-06-01)
We studied the role of galectin-3 (Gal-3) in the expression of alternative activation markers (M2) on macrophage, cytokines, and fibrosis through the temporal evolution of healing, ventricular remodeling, and function after myocardial infarction (MI). C57BL/6J and Gal-3 knockout mice (Lgals3-/-)
Effect of Flammulina velutipes on spent-hen breast meat tenderization.
Kang GH, et al.
Poultry Science, 91, 232-236 (2012)
Laura Antonucci et al.
International journal of oncology, 54(2), 505-514 (2018-11-30)
The aberrant activation of hedgehog (HH) signaling is a leading cause of the development of medulloblastoma, a pediatric tumor of the cerebellum. The FDA‑approved HH inhibitor, Vismodegib, which targets the transmembrane transducer SMO, has shown limited efficacy in patients with
Jerome C Servaites et al.
Analytical biochemistry, 421(1), 75-80 (2011-12-06)
Protein is a large component of the standing biomass of algae. The total protein content of algae is difficult to measure because of the problems encountered in extracting all of the protein from the cells. Here we modified an existing
One-step Coomassie brilliant blue R-250 staining of proteins in polyacrylamide gel.
H Chen et al.
Analytical biochemistry, 212(1), 295-296 (1993-07-01)
E Zeindl-Eberhart et al.
Electrophoresis, 18(5), 799-801 (1997-05-01)
A new, easy method for the immunodetection of specific antigens in two-dimensional electrophoresis (2-DE) is described. Areas of 2-DE gels containing antigens of interest are electrophoretically transferred to polyvinylidene difluoride membranes, immunostained with specific antibodies using Fast Red or 5-bromo-4-chloro-3-indolyl
Tomohito Kameda et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 47(13), 2035-2039 (2012-08-09)
The objective of this study is to evaluate the capacity of Mg-Al layered double hydroxide (Mg-Al LDH) intercalated with organic dye to adsorb aromatic compounds in aqueous solutions. Mg-Al LDH intercalated with Coomassie Brilliant Blue R anion (BB(-)) was prepared
Veronica Zaga-Clavellina et al.
Reproductive biology and endocrinology : RB&E, 9, 13-13 (2011-01-27)
Premature rupture of fetal membranes (PROM) complicated with intrauterine infection has been associated to alterations of the extracellular matrix (ECM) homeostasis. The aim of this work was to evaluate the integral/functional response of the amnion (AMN) and choriodecidua (CHD) to
Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green.
Shen Luo et al.
Analytical biochemistry, 350(2), 233-238 (2005-12-13)
Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this
Brandon Timm et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1150, 122162-122162 (2020-06-07)
The naturally extracellular hemoglobin (erythrocruorin) of the Canadian nightcrawler, Lumbricus terrestris (LtEc), is a unique oxygen transport protein that may be an effective substitute for donated human blood. Indeed, this ultra-high molecular weight (~3.6 MDa) hemoglobin has already been shown
C Scheler et al.
Electrophoresis, 19(6), 918-927 (1998-06-25)
Identification of proteins separated by two-dimensional electrophoresis (2-DE) is a necessary task to overcome the purely descriptive character of 2-DE and a prerequisite to the construction of 2-DE databases in proteome projects. Matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) has a
Laura R Kuck et al.
PloS one, 9(10), e109616-e109616 (2014-10-21)
Titer on Chip (Flu-ToC) is a new technique for quantification of influenza hemagglutinin (HA) concentration. In order to evaluate the potential of this new technique, a comparison of Flu-ToC to more conventional methods was conducted using recombinant HA produced in
Nadine Dyballa et al.
Methods in molecular biology (Clifton, N.J.), 893, 47-59 (2012-06-06)
Proteins separated by two-dimensional gel electrophoresis can be visualized by in-gel detection using -different staining methods. Ideally, the dye should bind non-covalently to the protein following a linear response curve. Since protein concentrations in biological systems may vary by six
Micaela Gliozzi et al.
International journal of molecular sciences, 21(20) (2020-10-15)
Clinical management of diabetic cardiomyopathy represents an unmet need owing to insufficient knowledge about the molecular mechanisms underlying the dysfunctional heart. The aim of this work is to better clarify the role of matrix metalloproteinase 2 (MMP-2) isoforms and of
Karel Mazanec et al.
Journal of mass spectrometry : JMS, 41(12), 1570-1577 (2006-12-01)
The use of low-molecular-mass color pI markers for the determination of pI values of proteins in gel isoelectric focusing (IEF) in combination with mass spectrometry is described. Different types of substituted phenols of known pI values within the mass range
Joseph M Tuscano et al.
Cancer immunology, immunotherapy : CII, 60(6), 771-780 (2011-02-25)
Previous studies have shown that bispecific antibodies that target both CD20 and CD22 have in vivo lymphomacidal properties. We developed a CD20-CD22 bispecific antibody (Bs20x22) from anti-CD20 and the anti-CD22 monoclonal antibodies (mAb), rituximab and HB22.7, respectively. Bs20x22 was constructed
Na Na et al.
Methods in molecular biology (Clifton, N.J.), 869, 511-520 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) has been one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. As a simple, convenient, and ultrasensitive detection of proteins for
Rozanne Arulanandam et al.
Communications biology, 3(1), 254-254 (2020-05-24)
We have demonstrated that microtubule destabilizing agents (MDAs) can sensitize tumors to oncolytic vesicular stomatitis virus (VSVΔ51) in various preclinical models of cancer. The clinically approved T-DM1 (Kadcyla®) is an antibody-drug conjugate consisting of HER2-targeting trastuzumab linked to the potent
Ye Dee Tay et al.
Cell reports, 26(6), 1654-1667 (2019-02-07)
NDR/LATS kinases regulate multiple aspects of cell polarity and morphogenesis from yeast to mammals. Fission yeast NDR/LATS kinase Orb6 has been proposed to control cell polarity by regulating the Cdc42 guanine nucleotide exchange factor Gef1. Here, we show that Orb6
Elias Leiva-Salcedo et al.
Mediators of inflammation, 2011, 152625-152625 (2011-09-24)
The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipopolysaccharide (LPS) is one of the strongest stimuli of the immune response, and
Elisa Agnese Nigro et al.
Scientific reports, 9(1), 16640-16640 (2019-11-14)
Polycystin-1 (PC-1) and 2 (PC-2) are the products of the PKD1 and PKD2 genes, which are mutated in Autosomal Dominant Polycystic Kidney Disease (ADPKD). They form a receptor/channel complex that has been suggested to function as a mechanosensor, possibly activated
K Kubo
Analytical biochemistry, 213(2), 200-205 (1993-09-01)
In sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Weber and Osborn, protein bands are often distorted by tailing at both ends, and the phenomenon is often called "edge tailing." This was eliminated by adding glycerol at
G Sreeramulu et al.
Electrophoresis, 16(3), 362-365 (1995-03-01)
A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (<
Marina Piscopo et al.
Hearing research, 178(1-2), 89-94 (2003-04-10)
The factors controlling otoconia growth are not well known but it seems that the type of proteins contained in the otoconia regulates the initiation and/or the subsequent rates of crystal growth determining the morphology and the size of the final
D Lee et al.
European journal of biochemistry, 268(2), 295-301 (2001-02-13)
alpha-Synuclein has been implicated in various neurodegenerative disorders, including Parkinson's and Alzheimer's diseases, by its participation in abnormal protein depositions. As the protein has been suggested to play a significant role in the formation of the deposits which might be
Dystrophin proteolysis: a potential target for MMP-2 and its prevention by ischemic preconditioning.
Bruno Buchholz et al.
American journal of physiology. Heart and circulatory physiology, 307(1), H88-H96 (2014-05-06)
Dystrophin is responsible for the mechanical stabilization of the sarcolemma, and it has been shown that it is one of the most sensitive proteins to ischemic injury. However, the enzyme responsible for this proteolysis is still unknown. Isolated rabbit hearts
Yong-Hak Kim et al.
Archives of biochemistry and biophysics, 494(2), 159-165 (2009-12-01)
We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was
Thaise L Teixeira et al.
Microbial pathogenesis, 135, 103618-103618 (2019-07-17)
P21 is a protein secreted by Trypanosoma cruzi (T. cruzi). Previous studies have shown a spectrum of biological activities performed by P21 such as induction of phagocytosis, leukocyte chemotaxis and inhibition of angiogenesis. However, the activity of P21 in T.
J A Beeley et al.
Electrophoresis, 17(3), 505-506 (1996-03-01)
Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially
G Houen et al.
Electrophoresis, 18(5), 701-705 (1997-05-01)
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of
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