D7440SIGMA

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Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

qPCR探针

qPCR探针利用荧光标记的靶特异性探针，从而提高了特异性和灵敏度。此外，还有多种荧光染料可供选择，因此可以使用多个引物同时扩增多个序列。

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

聚合酶链反应：

聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

热启动PCR

热启动PCR的目的在于抑制PCR反应，从而减少非特异性扩增、防止引物二聚体形成并提高产物产量。

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Elastase Function and Importance

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Papain, Cysteine Protease, Properties & Products

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

半胱氨酸蛋白酶——木瓜蛋白酶的特性和产品

木瓜蛋白酶是肽酶C1家族的一种半胱氨酸蛋白酶。它由单一多肽链组成，含有三个二硫键和一个酶活性所必需的巯基。

Nuclear Receptors (Non-Steroids)

We offer many products related to non-steroid nuclear receptors for your research needs.

Potassium Channels

We offer many products related to potassium channels for your research needs.

钾通道

我们可提供多种与钙通道相关的产品以满足您的各类研究需求。

DNA Damage and Repair

DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.

DNA损伤和修复

DNA损伤和修复机制对于维持DNA完整性至关重要。细胞DNA的损伤与突变、癌症发展等有关。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Discover Bioactive Molecules for Nucleotides

Cyclic nucleotides like cAMP modulate cell function via PKA activation and ion channels.