Functional genomics screening

应用筛选条件

facet applications:Functional genomics screening

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功能基因组学筛选_RNA干扰（RNAi）_CRISPR-Cas系统-默克生命科学

功能基因组学可以帮助人们分析发现基因的功能及其所参与的生化、细胞和生理学途径。得益于基因编辑、基因沉默、基因调节、下一代测序（NGS）和表型筛选技术的进步，研究人员可在多种模型系统中高效地进行功能基因组筛选。

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Antibiotic Optimization for Cell Transduction

Determine optimal antibiotic concentration for stable cell lines, ensuring efficient selection in transduced cells.

CRISPR Cas9 Proteins

The largest offering of quality Cas9 proteins ensures you have the perfect CRISPR reagents for your specific experimental needs. Rigorous purification, NLS signals, increased specificity and activity ensure your gene editing experiments are efficient and simple.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

The Researcher’s Guide to Designing CRISPR and RNAi Screening Experiments

This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.

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