Functional genomics screening

This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.

Experimental Design and Analysis — Experimental Design FAQs

We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.

esiRNA FAQs (Frequently Asked Questions)

esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Single-Cell Resolution Discovery

CRISPR lentiviral screening libraries, partnered with 10x Genomics, offer powerful research tools for pooled screening.

Examination of Lentiviral Library Efficiency

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

Next-Gen RNA Interference with MISSION^® shRNA

RNAi technology advancements enable gene expression silencing through endogenous RNAi pathways, applicable across eukaryotes.

CRISPR Cas9 Proteins

The largest offering of quality Cas9 proteins ensures you have the perfect CRISPR reagents for your specific experimental needs. Rigorous purification, NLS signals, increased specificity and activity ensure your gene editing experiments are efficient and simple.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Delivery of Nucleic Acids Using Polymers

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

siRNA Delivery in Mammalian Cell Lines

siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

Antibiotic Optimization for Cell Transduction

Determine optimal antibiotic concentration for stable cell lines, ensuring efficient selection in transduced cells.

miRNA (microRNA) Introduction

Mature microRNAs regulate gene expression through various mechanisms; partially complementary to mRNA molecules, downregulating gene expression.

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

MISSION® siRNA FAQs

We answer your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.

Nuclease-Free Water at Your Fingertips

An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.

Functional Genomics Screening

Functional genomics screening principles using RNAi and CRISPR/Cas9 libraries for drug target identification.

Mpox qPCR Products

Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.

Predesigned siRNA

Rosetta siRNA Design Algorithm optimizes siRNA sequences for reduced off-target effects and increased RNAi performance.