Nucleic acid gel electrophoresis

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facet applications:Nucleic acid gel electrophoresis

facet content type:Technical Article

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Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

DNA / Protein Electrophoresis and Troubleshooting Tables

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

Water for pH Measurement and Buffer Preparation

Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

Water for PCR Techniques

Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

DNA/蛋白质电泳及故障排除表

DNA和蛋白质凝胶电泳(SDS-PAGE)应用中的疑难问题解答。提供琼脂糖凝胶中DNA的有效分离范围和加样染料的DNA迁移大小。

Ultrapure vs. DEPC Treated Water for Molecular Biology Applications

Understand the benefits of ultrapure water versus DEPC treatment in DNA and RNA experiments to ensure high purity and integrity without risks.

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