Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.
Review resources about adding the Samplicity® filtration system to your HPLC workflow. Watch our step-by-step video to understand how the Samplicity® G2 filtration system works and use our application notes to learn more about how the Samplicity® G2 filtration system
Microscopy is an effective way to determine the nature, size, and source of contamination. In this section, you can find some detailed methods important for optical microscopy, as well as important considerations for scanning electron microscopy (SEM).
Millicup™-FLEX disposable vacuum filter was designed to reduce contamination and save time. The solvent-resistant unit allows for filtration into storage bottles.
HPTLC–MS analysis of quinine in tonic water and caffeine in coffee and cola drinks. Tonic and cola drinks decarbonized through ultrasonication, and coffee extracted through PTFE filter.
HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.
The B vitamins are very hydrophilic, consequently a gradient with a low concentration of methanol and acidic mobile phase was used for elution under reversed-phase conditions.