Protein and nucleic acid interactions

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Duolink® PLA Flow Cytometry Protocol

Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol

RNA immunoprecipitation (RIP) can identify specific RNA molecules of many types associated with specific nuclear or cytoplasmic binding proteins.

Handling and Storage Protocol for Synthetic Peptides

One of the most challenging aspects of working with synthetic peptides is determining the best solvent in which to dissolve the peptide. General guidelines for proper storage, handling and dissolving your synthetic peptide are provided.

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Duolink® PLA Multicolor Detection Protocol

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Using Duolink™ in Multiwell Plates

When using the Duolink reagents to stain cells grown in multiwell plates, a few modifications to the Duolink protocol, optimized for staining samples deposited on glass slides, have to be made in order to suit the multiwell format. In particular

Purification or Removal of DNA-Binding Proteins

This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.

Duolink® PLA Brightfield Protocol

This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

How to Create PLA® Probes

Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.

Chromatin Isolation by RNA Purification (ChIRP) Protocol

Chromatin Isolation by RNA purification (ChIRP) is a method for isolating regions of the genome that are bound by a specific RNA. This protocol uses magnetic beads coated with streptavidin to isolate the complex.