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抗体亲和色谱的脱盐和缓冲液置换
实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
Automated Antibody Purification with ÄKTA Systems
Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.
Performing a Separation with HiTrap® Protein G HP
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
IgG Antibody Purification with HiTrap® Protein G HP Columns
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.