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lichropur® bases for hplc and lc-ms
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App Note: Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in oat-based cereals
Methanol for UHPLC-MS LiChrosolv® 1.03726
Acetonitrile for UHPLC-MS LiChrosolv® 1.03725
Ammonia solution 25%, for HPLC LiChropur™ 5.43830
Formic acid for LC-MS LiChropur™, 97.5-98.5% (T) 00940
LC-MS Analysis of 33 PFAS Compounds in 5 minutes
8327 was completed in 5 minutes in this application note.
The HPLC column of choice for PFAS analysis by LC-MS/(MS) is a C18
column based on fully porous silica particles (FPP) or on superficially
porous
App Note: Charge Variant Analysis (CVA) with Strong Cation Exchange and MS Compatible Buffers
HPLC
Proteomix® SCX-NP5 (2.1 mm x 50) mm, 5 μm Z777156
Acetonitrile, LiChrosolv® hypergrade for LC-MS 1.00029
Water, LiChrosolv® for LC-MS 1.15333
Ammonium acetate, LiChropur™ for LC-MS
Brochure: LC-MS Resource Guide
Hexafluoro-2-propanol for LC-MS LiChropur™ 10 ml, 50 ml 18127
For a complete list of LC-MS and HPLC solvents,
buffers and additives, visit SigmaAldrich.com/lc-ms
LC-MS Tips and Tricks
Mobile
Reaction Monitoring of the Esterification Progress of Benzoic Acid
99.5% 242381
Acetonitrile hypergrade for LC-MS LiChrosolv® 1.00029
Water for chromatography (LC-MS Grade) LiChrosolv® 1.15333
Acetic acid for LC-MS LiChropur™ 5.33001
Reference
1. Maki, Takao;
Brighter Analysis - Solvents & Inorganics for accurate, brilliant results (RoW)
environmental analysis HPLC requires high-performance solvents and reagents.
Classical
Analysis
http://www.merckmillipore.com/Lichropur
LC-MS
Solvents
LC-MS
Reagents
Check
LC-MS Analysis of PFAS Compounds in EPA Methods 537.1, 533 and 8327
hypergrade for LC-MS LiChrosolv® 1.06035
Water for chromatography (LC-MS grade) LiChrosolv® or ultrapure water from a Milli-Q® IQ 7 series water purification system 1.15333
Ammonium acetate LiChropur™, eluent
Quantification of Psilocin and Psilocybin in Mushrooms
spectrometry (MS) 900667
Formic Acid, for LC-MS LiChropur™, 97.5-98.5% (T) 00940
Methanol, UHPLC, suitable for mass spectrometry
(MS) 900688
Water, for chromatography (LC-MS Grade) LiChrosolv
Brighter Analysis - Solvents & Inorganics for accurate, brilliant results
Analysis
Safety
&
Essentials
HPLC
&
LC-MS
Brighter
Analysis
LC-MSHPLC
HPLC
&
LC-MS
X
Analytix Reporter Issue 11 2022
Description Cat. No.
Ammonium formate, eluent additive for LC-MS,
LiChropur™, ≥99.0%
70221
Formic acid, for HPLC LiChropur™ 5.43804
Acetonitrile, gradient grade LiChrosolv® Reag. Ph Eur
Method Development for the Separation of Oligonucleotides Using Superficially Porous Particle Columns
ZIC®-HILIC 100Å (3.5 µm) HPLC
Columns, L × I.D. 100 mm x 2.1 mm HPLC
Column, PEEK-lined stainless steel
1504410001
Ammonium formate, eluent additive for LC-MS,
LiChropur™, ≥99.0% 70221-25G-F
Effect of Extracolumn Dispersion on Chromatographic Performance Using Narrow Bore UHPLC Columns
for MS
900667 Acetonitrile, for UHPLC, suitable for MS
G4505 Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent
00922 Difluoroacetic acid, for LC-MS, LiChropur™
00940
App Note: The use of DPX HLB tips and Hamilton® robot for extraction of opioid drugs from urine
phosphate dibasic heptahydrate ACS reagent, 98.0–102.0%
5.33003 Ammonia solution 25% for LC-MS LiChropur™
P5368 Phosphate buffered saline BioPerformance Certified, pH 7.4
52989-U SUPEL™ SWIFT
App Note: UHPLC-MS Bottom-Up Trastuzumab BIOshell™A160 Peptide C18
for MS 900682
Acetonitrile, for UHPLC, suitable for MS 900667
Guanidine hydrochloride, ≥99% (titration), organic base and chaeotropic agent G4505
Difluoroacetic acid, for LC-MS, LiChropur™ 00922
Peptide Mapping Analysis Workflow for Adalimumab
Water, LiChrosolv® for LC-MS 1153334000 Supelco®
Formic acid, LiChropur™ for LC-MS 533002 Supelco®
Accessories
Autosampler vials volume 0.3 mL 29661-U Supelco®
To place an order or receive
Flyer: Determination of Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and their metabolites in serum Hybrid SPE
Grade) LiChrosolv®
00940 Formic acid for LC-MS LiChropur™, 97.5-98.5% (T)
791725 Citric acid anhydrous, free-flowing, Redi-Dri™, ACS
reagent, ≥99.5%
52978-U HybridSPE® DPX tip
Z717266 NuncTM 96 DeepWell
Workflow for Intact and Middle-Up Mass Analysis of Adalimumab
or LiChrosolv® UHPLC-MS
grade bottled water
ZIQ7000T0
or 1.03728
Acetonitrile for UHPLC-MS LiChrosolv® 1.03725
Trifluoroacetic acid eluent additive for LC-MS,
LiChropur®
80457
Tris(2-
App Note: Analysis of Drugs of Abuse in Urine After Cleanup with New Supel™ Swift HLB Solid Phase Extraction 96-well Plates
methanol,
ampule of 1 mL, certified reference material, Cerilliant®
00940 Formic acid for LC-MS LiChropur™, 97.5-98.5% (T)
G8132 β-Glucuronidase from limpets (Patella vulgata) Type L-II,
lyophilized
Cannabis Potency Testing
Formic acid 98% - 100%, for LC-MS, LiChropur™ 5330020050
Acetonitrile with 0.1 % (v/v) formic acid for UHPLC, Sigma Aldrich 900686
Ammonium formate ≥99.0%, for LC-MS, LiChropur™ 70221
Ultrapure water
Application Note: Determination of PFAS in Drinking Water by LC-MS/MS according to GB 5750.8-2023
reagent, reag.
ISO, reag. Ph. Eur., ≥99.8%
33209/
33209-M
Ammonium acetate LiChropur™, eluent additive for
LC-MS
73594
Certified Reference Materials TraceCERT® (* unless otherwise
specified
App Note: Steroid Hormone Analysis in Serum using Supel™ Swift HLB DPX Tips
of 3 ea 53504-U
Formic acid 98-100%, LC-MS LiChropur™ 5.330002
Methanol, LC-MS LiChrosolv® 1.03726
Acetonitrile, LC-MS LiChrosolv® 1.00029
Water, LC-MS LiChrosolv® 1.15333
Suggested Consumables
Wallchart: Small Molecule HPLC Column Selection Guide
quality consumables for
the U/HPLC analysis workfl ow. Our selection
of columns, solvents, standards and sample
preparation products are designed for HPLC and
LC-MS to make your analysis quick, easy
Brochure: LC-MS Contaminants
acid in isopropanol at 0.2 mL/min for 48 min.
Overview of U/HPLC columns for LC-MS
For LC-MS, particulate HPLC columns, preferably based
on high purity Type B silica, are widely used.
1. Small particles
Low-extractable Millex Samplicity®filters: ideal for preparing samples for sensitive HPLC detectors
prior to HPLC analysis
should introduce minimal extractable impurities as
detected by a fluorescence detector. Similar to the
challenges with LC-MS, fluorescence intensity varies
based on the excitation
Ultrapure water for biomedical LC analysis
were analyzed using LC-ESI-MS-MS. High purity water was utilized and results highlight the
importance of low organic contamination to reach low levels of detection.
Denaturating HPLC is a reverse phase
Solutions for Mass Spectrometry
sensitive MS analyses, as it can help to avoid
handling based contaminations.
Water sample Intensity of reserpine
signal [cps]
LC-MS grade bottled water Competitor B 8.80 x 104
LC-MS grade
The Power to create better outcomes for your Research
Filter holders
Atomic Absorption / ICP
UV & IR Spectroscopy
Chromatography, HPLC, LC‐MS
Photometry
GC, GC‐MS,
GC headspace
Miscellaneous
NMR
Certipur® ICP
Standards, Certipur®
AAS
Poster: Rapid Sample Preparation Method for High Throughput Total Drug Analysis by LC-MS/MS
high recovery
No interfering extractable to LC-MS/MS or HPLC-UV
1000
10000
100000
1000000
10 50 100 500 1000
Analyte concentration (nM)
LC
-M
S/
M
S
ar
ea
(c
ou
nt
s)
Wallchart: Chiral HPLC Column Selection Guide
retention and selectivity. Typical
acid and base concentrations are 0.01
to 1%
• Acetonitrile should be the dominant
solvent with CYCLOBOND Column in POM
• LC-MS optimization: Replace TEA
with ammonium
MultiScreen Deep Well Solvinert Filter Plate: High throughput sample preparation for the quantitation of drug compounds in serum samples
following centrifugation.
LC-MS/MS analysis: Unless otherwise indicated, LC-MS/MS analyses were
performed using a Sciex API-2000 mass spectrometer coupled with an Agilent 1100
HPLC and well plate autosampler
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