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Campylobacter jejuni in penguins, Antarctica.
Petra Griekspoor et al.
Emerging infectious diseases, 15(5), 847-848 (2009-05-01)
Ben Long et al.
Frontiers in neuroscience, 14, 322-322 (2020-05-01)
Mapping the cytoarchitecture of the whole brain can reveal the organizational logic of neural systems. However, this remains a significant challenge, especially for gyrencephalic brains with a large volume. Here we propose an integrated pipeline for generating a cytoarchitectonic atlas
Chi Kin Ip
STAR protocols, 2(2), 100448-100448 (2021-04-30)
Chronic stress has adverse consequences on many organ systems and physiological processes. However, existing protocols show large variability in response and are not suitable for female mice. Here, we provide a step-by-step protocol for establishing a reliable chronic stress model
Compendium of Animal Rabies Prevention and Control, 2016.
Catherine M Brown et al.
Journal of the American Veterinary Medical Association, 248(5), 505-517 (2016-02-18)
James G Roberts et al.
ACS nano, 14(3), 2917-2926 (2020-02-15)
Carbon-fiber microelectrodes have proven to be an indispensable tool for monitoring exocytosis events using amperometry. When positioned adjacent to a cell, a traditional microdisc electrode is well suited for quantification of discrete exocytotic release events. However, the size of the
Zoltán Varga et al.
Colloids and surfaces. B, Biointerfaces, 192, 111053-111053 (2020-05-04)
Size characterization of extracellular vesicles (EVs) and drug delivery liposomes is of great importance in their applications in diagnosis and therapy of diseases. There are many different size characterization techniques used in the field, which often report different size values.
Peng Wan et al.
Neurophotonics, 8(3), 035007-035007 (2021-09-14)
Significance: The recently reported solvent-based optical clearing method FDISCO can preserve various fluorescent signals very well. However, the strict low-temperature storage condition of FDISCO is not conducive to long-time or repetitive imaging usually conducted at room temperature. Therefore, it is
Gizem Altay et al.
Bio-protocol, 10(3), e3514-e3514 (2021-03-04)
Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids' closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell
Bilal Rah et al.
Autophagy, 11(2), 314-331 (2015-03-25)
An active medicinal component of plant origin with an ability to overcome autophagy by inducing apoptosis should be considered a therapeutically active lead pharmacophore to control malignancies. In this report, we studied the effect of concentration-dependent 3-AWA (3-azido withaferin A)
Megan L Linscott et al.
PloS one, 14(7), e0220530-e0220530 (2019-07-31)
Fibroblast growth factor 8 (FGF8) is a potent morphogen that regulates the ontogenesis of gonadotropin-releasing hormone (GnRH) neurons, which control the hypothalamus-pituitary-gonadal (HPG) axis, and therefore reproductive success. Indeed, FGF8 and FGFR1 deficiency severely compromises vertebrate reproduction in mice and
Mano Sivaganesan et al.
BMC bioinformatics, 9, 120-120 (2008-02-27)
In real-time quantitative PCR studies using absolute plasmid DNA standards, a calibration curve is developed to estimate an unknown DNA concentration. However, potential differences in the amplification performance of plasmid DNA compared to genomic DNA standards are often ignored in
Yong N Li et al.
The Journal of comparative neurology, 516(5), 442-453 (2009-08-06)
Horizontal cells (HCs) are involved in establishing the center-surround receptive field organization of photoreceptor and bipolar cells. In many species, HCs respond differentially to colors and may play a role in color vision. An earlier study from our laboratory suggested
Wei Wang et al.
Stem cell research & therapy, 12(1), 99-99 (2021-02-05)
Reendothelialisation is the natural pathway that inhibits neointimal hyperplasia and in-stent restenosis. Circulating endothelial progenitor cells (EPCs) derived from bone marrow (BM) might contribute to endothelial repair. However, the temporal and spatial distributions of reendothelialisation and neointimal hyperplasia after EPC
Ioannis N Katis et al.
Biosensors & bioelectronics, 113, 95-100 (2018-05-09)
We report on the use of a laser-direct write (LDW) technique that allows the fabrication of lateral flow devices with enhanced sensitivity and limit of detection. This manufacturing technique comprises the dispensing of a liquid photopolymer at specific regions of
Dvir Gur et al.
Nature communications, 11(1), 6391-6391 (2020-12-16)
Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called
Agampodi Promoda Perera et al.
Scientific reports, 8(1), 8618-8618 (2018-06-07)
MCC950 a potent, highly specific small molecule inhibitor of canonical and noncanonical activation of NLRP3 inflammasome has been evaluated in a multitude of NLRP3 driven inflammatory diseases. However, the effect of MCC950 on colonic inflammation has not yet been reported.
S Vaddiraju et al.
Biosensors & bioelectronics, 24(6), 1557-1562 (2008-10-01)
The performance of an implantable glucose sensor is strongly dependent on the ability of their outer membrane to govern the diffusion of the various participating species. In this contribution, using a series of layer-by-layer (LBL) assembled outer membranes, the role
J Jason Hoth et al.
Shock (Augusta, Ga.), 31(4), 376-381 (2008-07-31)
Blunt chest trauma resulting in pulmonary contusion with an accompanying acute inflammatory response is a common but poorly understood injury. We previously demonstrated that toll-like receptor 2 (TLR-2) participates in the inflammatory response to lung injury. We hypothesized that the
Jennifer A-L M Pirotte et al.
The Journal of experimental biology, 221(Pt 20) (2018-09-13)
Winged aphids are described as hosts of lesser quality for parasitoids because a part of their resources is used to produce wings and associated muscles during their development. Host lipid content is particularly important for parasitoid larvae as they lack
Simon Dujardin et al.
Nature medicine, 26(8), 1256-1263 (2020-06-24)
Alzheimer's disease (AD) causes unrelenting, progressive cognitive impairments, but its course is heterogeneous, with a broad range of rates of cognitive decline1. The spread of tau aggregates (neurofibrillary tangles) across the cerebral cortex parallels symptom severity2,3. We hypothesized that the
Menglin Liu et al.
Nature communications, 10(1), 2126-2126 (2019-05-11)
Repair of the endothelial cell barrier after inflammatory injury is essential for tissue fluid homeostasis and normalizing leukocyte transmigration. However, the mechanisms of endothelial regeneration remain poorly understood. Here we show that the endothelial and hematopoietic developmental transcription factor Sox17
Courtney Choutka et al.
Autophagy, 13(9), 1573-1589 (2017-08-15)
The 2 main degradative pathways that contribute to proteostasis are the ubiquitin-proteasome system and autophagy but how they are molecularly coordinated is not well understood. Here, we demonstrate an essential role for an effector caspase in the activation of compensatory
Anna Iwaniak et al.
International journal of molecular sciences, 22(6) (2021-04-04)
In silico and in vitro methods were used to analyze ACE- and DPP-IV-inhibiting potential of Gouda cheese with a modified content of β-casein. Firstly, the BIOPEP-UWM database was used to predict the presence of ACE and DPP-IV inhibitors in casein
Tse-Kai Fu et al.
Marine drugs, 17(6) (2019-06-19)
More than 80% of infectious bacteria form biofilm, which is a bacterial cell community surrounded by secreted polysaccharides, proteins and glycolipids. Such bacterial superstructure increases resistance to antimicrobials and host defenses. Thus, to control these biofilm-forming pathogenic bacteria requires antimicrobial
R Alvarez et al.
Journal of virology, 82(1), 471-486 (2007-10-19)
Understanding why human immunodeficiency virus (HIV) preferentially infects some CD4(+) CD45RO(+) memory T cells has implications for antiviral immunity and pathogenesis. We report that differential expression of a novel secreted factor, ps20, previously implicated in tissue remodeling, may underlie why
Seyi D Eletu et al.
Access microbiology, 2(3), acmi000094-acmi000094 (2020-09-26)
National surveillance of pneumococcal disease at the serotype level is essential to assess the effectiveness of vaccination programmes. We previously developed a highly sensitive extended-specificity multiplex immunoassay for detection of Streptococcus pneumoniae serotype-specific antigen in urine in the absence of
Can Zhou et al.
Biomedical optics express, 11(12), 7132-7149 (2021-01-08)
Optical clearing methods are widely used for three-dimensional biological information acquisition in the whole organ. However, the imaging quality of cleared tissues is often limited by ununiformed tissue clearing. By combining tissue clearing with mechanical sectioning based whole organ imaging
Russell Dibb et al.
Magnetic resonance in medicine, 77(6), 2331-2346 (2016-07-08)
To develop a susceptibility-based MRI technique for probing microstructure and fiber architecture of magnetically anisotropic tissues-such as central nervous system white matter, renal tubules, and myocardial fibers-in three dimensions using susceptibility tensor imaging (STI) tools. STI can probe tissue microstructure
W L Ung et al.
Lab on a chip, 17(23), 4059-4069 (2017-10-11)
We demonstrate an acoustic wave driven microfluidic cell sorter that combines advantages of multilayer device fabrication with planar surface acoustic wave excitation. We harness the strong vertical component of the refracted acoustic wave to enhance cell actuation by using an
Patricia A Trimmer et al.
Molecular neurodegeneration, 4, 26-26 (2009-06-19)
It has been hypothesized that reduced axonal transport contributes to the degeneration of neuronal processes in Parkinson's disease (PD). Mitochondria supply the adenosine triphosphate (ATP) needed to support axonal transport and contribute to many other cellular functions essential for the
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