CN
- Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.
Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.
Chemical communications (Cambridge, England) (2013-01-26)
Shota Morimoto, Takenori Tomohiro, Nobuyuki Maruyama, Yasumaru Hatanaka
PMID23349004
摘要
A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.