Supel™ Carbon LC 2.7 µm 100 x 3.0 mm I.D. (59993-U)
[A] Water + 0.1% TFA,[B] Acetonitrile + 0.1% TFA
0-0.5 min 2.5% B, in 4.5 min to 95% B, 2 min 95% B, 3 min at 2.5% B
0.5 mL/min
80 – 130 bar
90 °C
UV, 230 nm
5 µL
six nitrosamines standard mix (~20 mg/L each) in mobile phase A (water + 0.1% TFA)
Nitrosamines have been known to exist in the environment and within food sources, with awareness further raised in 2018 when many regulatory authorities for medicines became aware of the dangers of N-Nitrosamines as drug contaminants. This class of compounds is known to be potent carcinogens and can be introduced through multiple introduction pathways. In this application, 2.7 µm porous graphitic carbon particles in Supel™ Carbon LC column are used to separate six different nitrosamines, followed by UV detection.
The Supel™ Carbon LC column is used for the separation of six nitrosamine compounds. The column is able to separate six nitrosamines based on reversed phase with good reproducibility for repeated analyses (data not shown here). The resolution and peak asymmetry for the six different nitrosamines are greater than 1.5 and less than 2.0, respectively. The LOD and LOQ of the tested nitrosamines are in the low mg/L range and can be further improved with the use of mass spectrometry instrumentation.
APP_661
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