HPLC Analysis of Sulfonamide Positional Isomers on Discovery^® Zr-Carbon C18

column

Discovery Zr-Carbon C18, 15 cm × 4.6 mm I.D., 3 μm particles (65706-U)

mobile phase

[A] 10mM diethylamine, pH 10.8; [B] acetonitrile;

flow rate

1.5 mL/min

column temp.

80 °C

detector

UV, 240 nm

injection

5 μL