LC/MS Analysis of Triglycerides in Soybean Oil on Ascentis^® C18

column

two Ascentis C18, 15 cm × 2.1 mm I.D., 3 μm particles connected in series (581302-U)

mobile phase

[A] 2-propanol: [B] acetonitrile; (60:40, A:B)

flow rate

0.2 mL/min

column temp.

25 °C

detector

MS APCI mode

injection

2 μL

sample

soybean oil weighed and diluted in acetone (10 mg in 1 ml w/v)