LC/MS Analysis of Triclosan (Irgasan) in Waste Water on Ascentis® Express C18 after SPE using Supel™-Select HLB
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
waste-water treatment plant effluent
SPE tube/cartridge
Supel Select HLB 60 mg/ 3 mL (54182-U)
condition
3 mL methanol, 3 mL distilled water, 3 mL 0.01 M HCl
sample addition
50 mL
washing
wash using 10 mL distilled water, dry for 5 minutes under vacuum
elution
3 ml acetonitrile:methanol (1:1)
eluate post-treatment
evaporated to dryness and reconstituted into the HPLC mobile phase
column
Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)
mobile phase
[A] methanol; [B] acetonitrile; [C] 0.1% formic acid; (40:40:20, A:B:C)
flow rate
0.15 mL/min
pressure
1885 psi (130 bar)
column temp.
30 °C
detector
ESI(-), MRM, 286.8/35.0
injection
10 μL
说明
分析说明
Triclosan is one of the most prevalent antibacterial compounds added to many consumer products. It ends up in surface and ground waters from municipal waste water treatment plants, among other routes. The compound is under scrutiny because of its link to a wide range of health effects, including skin irritation and endocrine disruption, its impact on fragile aquatic biosystems, and its promotion of antibiotic resistance. Analysis of triclosan in waste water plant effluent using Supel-Select HLB SPE cleanup and LC/MS/MS using Ascentis Express 2.7 μm C18 column is shown here.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC