Protease Inhibitors - Don't Lose Your Proteins During Sample Prep

Protect Your Proteins

Protease inhibitors are valuable and useful reagents for researchers who want to inhibit general degradation of proteins in tissue or cell extracts by endogenous proteases. During isolation and characterization, proteases may pose a threat to the fate of a protein.

Four main classes of proteolytic enzymes are routinely utilized to describe proteases:

• Serine Protease Class includes trypsin, chymotrypsin and elastase.
• Cysteine Protease Class includes papain, calpain and lysosomal cathepsins.
• Aspartic Protease Class includes pepsin and rennin
• Metallo-protease Class include thermolysin and carboxypeptidase A

During isolation and characterization, one or all four classes of proteases may pose a threat to the fate of a protein. Broad spectrum protease inhibitors and cocktails (mixtures) have been developed to protect the integrity of proteins against multiple classes of proteases.

Protease inhibitor cocktails have been developed to safeguard the integrity of proteins during extraction and purification, to protect your proteins during sample prep. Sigma-Aldrich offers the broadest range of well-established individual protease inhibitors and protease inhibitor cocktails on the market. In addition to the Sigma-Aldrich line of protease inhibitor cocktails, our product offerings now include Roche’s cOmplete protease inhibitor cocktail tablets.

Merck offers the broadest range of well-established individual protease inhibitors and protease inhibitor cocktails that are optimized to maintain and preserve protein functionality following cell lysis.

• Product offerings now include Roche’s cOmplete protease inhibitor cocktail tablets
• Available in powder, solution, or tablet form
• Inhibits proteases in almost any sample type (tissue or cell)
• Specific formulations optimized for particular applications

View our individual protease inhibitors at
sigma-aldrich.com/proteaseinhibitors

View our protease inhibitor cocktails at
sigma-aldrich.com/proteaseinhibitorscocktails

Find individual protease inhibitors at
sigma-aldrich.com/proteaseinhibitors