产品名称
细胞游离氨基酸混合物 - 13C,15N, 5-100 mM in water, 98 atom % 15N, 98 atom % 13C
isotopic purity
98 atom % 13C
98 atom % 15N
form
liquid
concentration
5-100 mM in water
technique(s)
bio NMR: suitable
protein expression: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
Analysis Note
氨基酸浓度因批次而异。请参见特定批次检验报告。
近似浓度（mM）：
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20
近似浓度（mM）：
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20
Application
该产品适合作为无细胞蛋白质合成的稳定同位素标记氨基酸的来源。对于质谱应用，考虑产品909653，用于质谱的稳定同位素标记氨基酸混合物。
由于酪氨酸的浓度相对较高，产品可能会出现浑浊。一旦稀释使用，溶液应澄清。
由于酪氨酸的浓度相对较高，产品可能会出现浑浊。一旦稀释使用，溶液应澄清。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Preparation of amino acid mixtures for cell-free expression systems
Caschera, F., Noireaux, V
Biotechniques, 58, 40-43 (2015)
Estelle Couradeau et al.
Nature communications, 10(1), 2770-2770 (2019-06-27)
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare
Zhenzhen Liu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1124, 72-81 (2019-06-10)
A simple and fast LC-MS/MS method was developed and validated for simultaneous quantification of 20 proteinogenic l-amino acids (AAs) in a small volume (5 μL) of mouse plasma. Chromatographic separation was achieved on an Intrada Amino Acid column within 13 min via
Filippo Caschera et al.
BioTechniques, 58(1), 40-43 (2015-01-22)
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all
Sara Calhoun et al.
Communications biology, 4(1), 333-333 (2021-03-14)
Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus
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