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Merck
CN

ESG1106

ESGRO®重组小鼠LIF蛋白

ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml

别名:

Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32160801
Form:
liquid
Assay:
>95% (active component mLIF, SDS-PAGE)

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assay

>95% (active component mLIF, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1000000 units/mL

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

NCBI accession no.

NM_008501

UniProt accession no.

P09056

shipped in

wet ice

Quality Level

100

Gene Information

mouse ... LIF(16878)

相关类别

General description

ESGRO 小鼠 LIF 培养基补充剂以有效单位/mL 的形式提供，以确保批次之间的性能一致。 每批 ESGRO 补充剂均经过严格的质量控制测试，以确保能可靠地抑制小鼠多能干细胞分化。 ESGRO 小鼠 LIF 补充剂可用于小鼠胚胎干（ES）和诱导性多能干（iPS）细胞的基于饲养层和无饲养层的培养。
小鼠白血病抑制因子（LIF，mLIF）通过抑制自发分化，促进多能小鼠胚胎干（ES）和诱导多能干（iPS）细胞的自我更新和长期维持。

Application

ESGRO 白血病抑制因子（LIF）补充剂，用于小鼠 ES 细胞培养。 每小瓶含有 10^6 单位/ml
ESGRO 补充剂的应用包括用作体外维持鼠 ES 细胞多能表型的试剂。

建议的实验方案：

ES 细胞：在 D3 和 MBL-1 多能 ES 细胞培养物中，研究发现，通常每 1.0 mL 组织培养基需要 1000 U ESGRO 补充剂来维持具有干细胞表型的 ES 细胞。 相似浓度的 mLIF 也已用于遗传修饰的 ES 细胞的种系传递（E3）。

在推荐浓度下，107 单位的 ESGRO 补充剂足以用于 10.0 L 的组织培养基，而 106 单位的 ESGRO 补充剂足以用于 1.0 L 的组织培养基。

Physical form

ESGRO 补充剂以液体形式提供，含量为 106 单位，溶于 1.0 mL 磷酸盐缓冲盐水，含 1％w/v 牛血清白蛋白 BSA 为载体以确保稳定性。
形式：纯化

Preparation Note

ESGRO 补充剂在环境温度下运输。出于客户关系，可能包含一个冰袋，但确实没必要。开发期间已使用 ESGRO 补充剂进行了广泛的稳定性测试。 该产品自生产之日起，以浓缩形式或在无菌组织培养基中稀释后至少可稳定保存 18 个月，且对 ES 细胞没有活性损失。 若要长期储存，建议将 ESGRO 补充剂储存于 4°C。 冻融会降低效力。建议在使用前，将 ESGRO 补充剂在无菌组织培养基中稀释并等分至合适的浓度，然后储存于 4°C。应避免冻融。在 ES 细胞培养期间，该产品在 37°C，5％ CO2 培养箱中可稳定至少 7 天。
提供的 ESGRO® 补充剂经 0.22 微米无菌过滤，并在支原体检测中呈阴性。

Analysis Note

比活度：ESGRO® 补充剂在小鼠胚胎干细胞（D3 & MBL-1）和鼠 M1 髓样白血病细胞上进行了评估（4）。50 单位的标准定义为在 1.0mL 组织培养基中可诱导 50％ 的 M1 菌落分化的 ESGRO 补充剂的浓度（4）。 106 单位相当于大约 10 μg 的纯蛋白质，足以处理 1L 的 ES 细胞培养基。

胚胎干细胞测定：分化抑制测定，浓度为 1000 单位/mL 小鼠骨髓白血病细胞 M1：比活度 >/= 108 单位/mg

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Systematic delineation of optimal cytokine concentrations to expand hematopoietic stem/progenitor cells in co-culture with mesenchymal stem cells.
Andrade, Pedro Z, et al.
Molecular Biosystems, 6, 1207-1215 (2010)
Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification.
Ito S. et al.
Nature null
Ashley L Fritz et al.
Biotechnology and bioengineering, 112(1), 209-219 (2014-07-30)
The induction of pluripotency from adult cells has enormous potential in regenerative medicine. While initial efforts to study mechanisms and improve efficiency of induced pluripotent stem cell (iPSC) reprogramming focused on the direct roles of transcriptional regulators, increasing evidence indicates
Differentiation of Radial Glia-Like cells from embryonic Stem Cells
Liour, S and Yu, RK.
Glia, 42(2), 109-117 (2003)
Brandon Decker et al.
International journal of molecular sciences, 22(1) (2021-01-06)
During the development of mouse embryonic stem cells (ESC) to neuronal committed cells (NCC), coordinated changes in the expression of 2851 genes take place, mediated by the nuclear form of FGFR1. In this paper, widespread differences are demonstrated in the
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