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Merck
CN

EZMCP1

Human MCP-1 ELISA Kit

detects MCP-1 in 50 μL serum, plasma or cell culture supernatent

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UNSPSC Code:
41116158
NACRES:
NA.84

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产品名称

Human MCP-1 Conferma® ELISA, quantification of human MCP-1 in serum, plasma, or culture media samples.

clone

monoclonal

species reactivity

human

parameter

50 μL sample volume (4hr assay)

assay range

linearity: 94-106 (serum)%
linearity: 94-110 (plasma)%
inter-assay cv: <12.9%
intra-assay cv: <4.9%
standard curve range: 1.17-300 pg/mL
accuracy: 84-95%

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

UniProt accession no.

P13500

application(s)

research use

detection method

colorimetric (450nm/590nm)

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... CCL2(6347)

相关类别

Analysis Note

3 hour 45 minute assay, room temperature, 1:5 dilution recommended e.g.30 μL sample + 120μL of diluent (serum, plasma, or tissue culture medium)

Application

This Human MCP-1 Conferma® ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Disclaimer

For research use only. Not for use in diagnostic procedures.

General description

Human MCP-1 is a chemokine assoscited with the attraction of Monocytes and Basophils to the site of a pathology. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. In particilar this is a marker of inflammatory disorders. It binds to chemokine receptors CCR2 and CCR4.
This is a 96 well format sandwich ELISA which uses our Conferma® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Packaging

Two boxes; each to be analyzed separately.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

存储类别

8A - Combustible, corrosive hazardous materials

wgk

WGK 3

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商品

Conferma® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

实验方案

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

ELISA实验方法

该文章提供了4种常用的ELISA实验方法：夹心法ELISA实验方法、磷酸化检测过程、EIA检测过程以及基于细胞的检测过程

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