IPVH00010
Immobilon® -P PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane
Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
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About This Item
Product Name
Immobilon®-P PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
material
PVDF membrane
plain filter
white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable
western blot: suitable
filter L × W
27 cm × 3.75 m
pore size
0.45 μm pore size
capacity
160 μg/cm2 adsorption capacity (insulin)
215 μg/cm2 adsorption capacity (BSA)
294 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination
detection method
chemiluminescent
colorimetric
fluorometric
radioactive
shipped in
ambient
Quality Level
Features and Benefits
- Won′t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times
General description
Other Notes
Legal Information
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Discover cell culture applications for hormones including insulin and dexamethasone. Explore high-quality supplements.
比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。
Protocols
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。
Related Content
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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