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Merck
CN

IPVH07850

Immobilon® -P PVDF Membrane

50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, transfer membrane

别名:

Western blotting membrane, blotting membrane, mini blots, transfer membrane

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UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

主要文件

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产品名称

Immobilon®-P PVDF转印膜, 50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

Quality Level

400

相关类别

Application

Immobilon®-P PVDF已用于蛋白质印迹分析。

Features and Benefits

Immobilon®-P膜特点：
  • 出色的蛋白质保留率
  • 高物理强度
  • 广泛的化学相容性
  • 适用各种染色应用及免疫检测方法再生检测

General description

Immobilon®-P转印膜是一种聚偏氟乙烯(PVDF)微孔膜，标称孔径为0.45微米(μm)。推荐用于蛋白质印迹分析(western blotting)，尤其是大于20 kDa的蛋白质。Immobilon®-P转印膜可结合各种分子量的蛋白质。用于从各种凝胶基质中转出蛋白质。与硝酸纤维素膜相比，Immobilon-P PVDF膜信噪比更高、更灵敏，处理和染色性能更佳，溶剂耐受性也有加强。
主要吸附作用：静电、疏水；
过滤器代码：IPVH

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Paola Maycotte et al.
Autophagy, 8(2), 200-212 (2012-01-19)
Chloroquine (CQ) is a 4-aminoquinoline drug used for the treatment of diverse diseases. It inhibits lysosomal acidification and therefore prevents autophagy by blocking autophagosome fusion and degradation. In cancer treatment, CQ is often used in combination with chemotherapeutic drugs and
Rong Kang et al.
American journal of otolaryngology, 33(6), 678-684 (2012-07-10)
The purposes of this study were to determine whether autophagy was involved in cisplatin (CDDP) resistance and to investigate the role of the autophagy in the regulation of chemosensitivity to CDDP in laryngeal cancer Hep-2 cells. A WST-1 assay was
Sheikh F Ahmad et al.
Neurotoxicology, 77, 1-11 (2019-12-08)
Autism spectrum disorder (ASD) comprises a broad range of neurodevelopmental disorders that are associated with deficits in social interaction and communication. The tyrosine kinase inhibitor tyrphostin AG126 represents a promising therapeutic agent for several neuroinflammatory disorders. There are currently no
Kentaro Togashi et al.
Journal of the neurological sciences, 413, 116802-116802 (2020-04-04)
Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc). Levodopa (L-Dopa), the current main treatment for PD, reduces PD symptoms by partially replacing dopamine, but it does not slow neurodegeneration. Recent

实验方案

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌制PAGE和SDS-PAGE凝胶

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

相关内容

Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

SNAP i.d. 2.0 System Brochure

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

Immobilon -P Blotting Sandwiches
Immobilon®-P Transfer Membrane User Guide
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