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Merck
CN

MMA205

Millipore

Millicell® µ-迁移检测试剂盒

This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear & lasts for more than 48 hours.

别名:

microfluidic cellular migration assay kit

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UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NB.13
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种属反应性

rat, mouse, human

制造商/商品名称

Millicell®

技术

cell based assay: suitable
single cell analysis: suitable

运输

dry ice

一般描述

  • 细胞迁移发生在各种生物过程中,包括伤口愈合、胚胎发育和免疫反应。由于细胞迁移在血管生成、肿瘤学、神经病学——尤其是免疫学——中的重要性,人们对在单细胞水平上了解潜在生物机制的兴趣日益浓厚。
  • 传统的多孔(博伊登室)迁移测定可能存在诸多局限,例如:(1)梯度不是线性的,公认的或受控的;(2)迁移细胞的定量是基于终点测量而不是动态确定的;(3)目前无法对迁移时的细胞进行成像。
  • Millicell µ-迁移测定试剂盒克服了这些限制。
  • 其微流体、低容量技术可促进稳定的、由扩散产生的浓度梯度,该梯度始终是线性的,可持续48小时以上。µ-迁移载玻片设计用于视频显微镜分析,由具有与玻璃相似的高光学质量的塑料制成。在特定的时间间隔内,可获取观察区域的图像,实时监测和定量测定细胞迁移。

特点和优势

  • 单细胞或细胞群 迁移的活细胞追踪
  • 建立稳定且线性 的浓度梯度,持续≥ 48 小时
  • 有助于区分趋化性和 随机运动
  • 允许进行多参数分析, 包括细胞方向性和速度
  • 增强慢速和 快速迁移细胞的光学成像
  • 并行分析 3 种趋化剂, 以提高通量和灵活性
  • 无需花时间或金钱进行 优化
  • 即用型,无需组装

制备说明

1.Millicell® µ-迁移玻片(部件号CS204500)。室温储存。
2.Millicellµ-迁移帽和插头(部件号CS204501)在室温下储存。
3.人IV型胶原(部件号CS204498)储存于-20ºC。
4.醋酸缓冲液(部件号CS204485)在室温下储存。
5.BSA溶液(部件号CS204507)储存于-20ºC。
6.斜面移液器吸头。室温储存。

其他说明

  1. Millicell® µ-迁移玻片:(部件号CS204500)4个无涂层灭菌玻片。每张玻片包含3个腔室。
  2. Millicell® µ-迁移帽和塞:(部件号CS204501)各48个。每个载玻片12个盖子和12个塞子。
  3. 人IV型胶原: (部件号CS204498)一瓶100 µg的1 mg/mL人胶原IV。
  4. 乙酸缓冲液:(部件号CS204485)一个装有1.5 mL 0.5 M乙酸的小瓶。
  5. BSA溶液:(部件号CS204507)一个装有100 µL 30% BSA溶液的小瓶。
  6. 斜面移液器吸头: Fisherbrand SureOne 200 µL吸头,Fisher目录号02-707-419。两个96头盒。

法律信息

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

储存分类代码

10 - Combustible liquids


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Qiao Liao et al.
Acta pharmacologica Sinica, 40(10), 1314-1321 (2019-05-08)
Irisin, a myokine, is cleaved from the extracellular portion of fibronectin domain-containing 5 protein in skeletal muscle and myocardium and secreted into circulation as a hormone during exercise. Irisin has been found to exert protective effects against lung and heart injuries.

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