MMA205
Millicell® µ-迁移检测试剂盒
This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear & lasts for more than 48 hours.
别名:
microfluidic cellular migration assay kit
种属反应性
rat, mouse, human
制造商/商品名称
Millicell®
技术
cell based assay: suitable
single cell analysis: suitable
运输
dry ice
一般描述
- 细胞迁移发生在各种生物过程中,包括伤口愈合、胚胎发育和免疫反应。由于细胞迁移在血管生成、肿瘤学、神经病学——尤其是免疫学——中的重要性,人们对在单细胞水平上了解潜在生物机制的兴趣日益浓厚。
- 传统的多孔(博伊登室)迁移测定可能存在诸多局限,例如:(1)梯度不是线性的,公认的或受控的;(2)迁移细胞的定量是基于终点测量而不是动态确定的;(3)目前无法对迁移时的细胞进行成像。
- Millicell µ-迁移测定试剂盒克服了这些限制。
- 其微流体、低容量技术可促进稳定的、由扩散产生的浓度梯度,该梯度始终是线性的,可持续48小时以上。µ-迁移载玻片设计用于视频显微镜分析,由具有与玻璃相似的高光学质量的塑料制成。在特定的时间间隔内,可获取观察区域的图像,实时监测和定量测定细胞迁移。
特点和优势
- 单细胞或细胞群 迁移的活细胞追踪
- 建立稳定且线性 的浓度梯度,持续≥ 48 小时
- 有助于区分趋化性和 随机运动
- 允许进行多参数分析, 包括细胞方向性和速度
- 增强慢速和 快速迁移细胞的光学成像
- 并行分析 3 种趋化剂, 以提高通量和灵活性
- 无需花时间或金钱进行 优化
- 即用型,无需组装
制备说明
2.Millicellµ-迁移帽和插头(部件号CS204501)在室温下储存。
3.人IV型胶原(部件号CS204498)储存于-20ºC。
4.醋酸缓冲液(部件号CS204485)在室温下储存。
5.BSA溶液(部件号CS204507)储存于-20ºC。
6.斜面移液器吸头。室温储存。
其他说明
- Millicell® µ-迁移玻片:(部件号CS204500)4个无涂层灭菌玻片。每张玻片包含3个腔室。
- Millicell® µ-迁移帽和塞:(部件号CS204501)各48个。每个载玻片12个盖子和12个塞子。
- 人IV型胶原: (部件号CS204498)一瓶100 µg的1 mg/mL人胶原IV。
- 乙酸缓冲液:(部件号CS204485)一个装有1.5 mL 0.5 M乙酸的小瓶。
- BSA溶液:(部件号CS204507)一个装有100 µL 30% BSA溶液的小瓶。
- 斜面移液器吸头: Fisherbrand SureOne 200 µL吸头,Fisher目录号02-707-419。两个96头盒。
法律信息
免责声明
储存分类代码
10 - Combustible liquids
相关内容
"The successful, reliable culture of epithelial cells is critical for many areas of research, including dermatology, respiratory research, and cancer research. Because the breakdown of control mechanisms in epithelial cells is a frequent contributor to cancer progression and metastasis, epithelial cell culture is particularly important for cancer research. EpiGRO™ media formulations are optimized to provide better viability, proliferation rates, morphology and culture stability than other commercially available options. The media are provided in unique, light-blocking, temperature-monitored packaging to ensure stability and protect the media from damage by light, contamination, and excessive heat. The media do not require or contain any antimicrobials or phenol red. These components can cause cell stress and influence experimental results by masking the true performance or health of the cell culture. Phenol red acts like an estrogen and may stimulate growth independently of experimental variables. "
Cell migration is stimulated and directed by interaction of cells with the extracellular matrix (ECM), neighboring cells, or chemoattractants. Cell migration participates in morphogenic processes, wound healing and tumor metastasis. Specifically, inhibiting tumor invasion by blocking tumor cell chemotaxis has been a major focus of research. Tumor cell invasion, marked by degradation of ECM, is also directly correlated with metastatic potential.
"Recognizing both the tremendous opportunities and the challenges facing cancer research, we are dedicated to developing and refining tools and technologies for the study of cancer. With our comprehensive portfolio, including the Upstate®, Chemicon®, and Calbiochem® brands of reagents and antibodies, researchers can count on dependable, high quality solutions for analyzing all the hallmarks of cancer."
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