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Merck
CN

SF006

ESGRO Complete 细胞消化液

The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

别名:

Stem Cell Tested Accutase

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UNSPSC代码:
12352207
eCl@ss:
32160801
NACRES:
NA.75

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质量水平

100

表单

liquid

制造商/商品名称

Chemicon®

技术

cell culture | stem cell: suitable

输入

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

运输

dry ice

一般描述

ESGRO Complete™ Accutase 是一种蛋白水解和胶原分解酶的细胞分离溶液，经确认可用于分离在无血清条件下用ESGRO Complete 克隆级培养基（目录号SF001-500）培养的小鼠胚胎干细胞。 Accutase 不含有哺乳动物或细菌来源产品。

应用

细胞分离：

1.将Accutase®在室温下解冻。

2.用无菌PBS洗涤孔板、烧瓶或珠子。

3.通过无菌程序添加Accutase至培养皿或烧瓶中（每75平方厘米表面积10 mL）。

4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。

5.按常规操作进行细胞计数和传代。不需要额外的洗涤或酶抑制剂。

外形

冷冻无菌液体，即用型制剂。 每批都已经过无菌性（通过USP膜过滤方法）、酶活性（通过合成发色四肽测试）和细胞与组织培养塑料的分离性能测试。

1X accutase® 酶，溶于含0.5 mM EDTA•4Na和3 mg/L苯酚红的Dulbecco′s PBS中。

制备说明

保存于-20°C可保持稳定。请参阅标签上的批次失效日期。 建议收货时保存于-20°C。 解冻后，Accutase® 可以在4°C下最多保存2个月。请勿在室温下存放。

法律信息

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Na Suo et al.
Glia, 67(7), 1320-1332 (2019-03-01)
Oligodendrocytes (OLs) are the myelinating glia of the central nervous system. Injury to OLs causes myelin loss. In demyelinating diseases, such as multiple sclerosis, the remyelination is hindered principally due to a failure of the oligodendrocyte precursor cells (OPCs) to
Thangaselvam Muthusamy et al.
Stem cell reports, 3(1), 169-184 (2014-07-30)
We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of
Lina Dahl et al.
PloS one, 3(4), e2025-e2025 (2008-04-24)
The molecular mechanisms regulating the expansion of the hematopoietic system including hematopoietic stem cells (HSCs) in the fetal liver during embryonic development are largely unknown. The LIM-homeobox gene Lhx2 is a candidate regulator of fetal hematopoiesis since it is expressed
Darren A Cusanovich et al.
Cell, 174(5), 1309-1324 (2018-08-07)
We applied a combinatorial indexing assay, sci-ATAC-seq, to profile genome-wide chromatin accessibility in ∼100,000 single cells from 13 adult mouse tissues. We identify 85 distinct patterns of chromatin accessibility, most of which can be assigned to cell types, and ∼400,000
Peiyao A Zhao et al.
Genome biology, 21(1), 76-76 (2020-03-27)
DNA replication in mammalian cells occurs in a defined temporal order during S phase, known as the replication timing (RT) programme. Replication timing is developmentally regulated and correlated with chromatin conformation and local transcriptional potential. Here, we present RT profiles
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相关内容

Feeder-free and Serum-free ES Cell Culture using ESGRO Complete System
Derivation of Mouse Embryonic Stem Cells in Serum-Free and Feeder-Free Conditions
ESGRO Complete Products for Mouse Embryonic Stem Cell Culture in Serum-Free and Feeder-Free Conditions
Murine Embryonic Stem Cell Culture Procedures & Protocols

The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.

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