产品名称
细胞毒性检测试剂盒(LDH), suitable for protein quantification, suitable for cell analysis, detection, sufficient for ≤2,000 tests
usage
sufficient for ≤2,000 tests
packaging
pkg of 1 kit
manufacturer/tradename
Roche
technique(s)
protein quantification: suitable
λmax
490 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
−20°C
Quality Level
Application
细胞毒性检测试剂(LDH)是一种基于96孔板形式对来自受损细胞释放的LDH活性进行快速而简单测量的方法,用于对细胞毒性/细胞裂解进行量化。因此,当对细胞质膜的损伤发生时,该试剂盒可用于许多不同体外细胞系统。例如:
- 细胞介导的细胞毒性检测和定量
- 调解因子介导的细胞裂解测定
- 测定化合物在环境和医学研究中细胞毒性潜能,应用于食品、化妆品及制药行业
- 测定生物反应器中的细胞死亡
- 检测在骨组织工程中用于骨细胞生长的多种支架的生物兼容性
General description
作为[51Cr]以及[3H]-胸苷释放检测的一种非放射性替代方法。
比色检测用于细胞死亡和细胞裂解的定量,基于对从受损细胞胞液释放至上清中的乳酸脱氢酶(LDH)进行测定。
比色检测用于细胞死亡和细胞裂解的定量,基于对从受损细胞胞液释放至上清中的乳酸脱氢酶(LDH)进行测定。
Other Notes
Packaging
1个试剂盒包含2种组分。
Preparation Note
工作溶液:瓶1催化剂:
用1 ml双蒸水将冻干酶重悬10分钟并充分混匀。
瓶2染料溶液:
即用型溶液。
反应混合物
用于100次检测:在使用前不久,将瓶1中的250 μl与瓶2中的11.25 ml的进行混合。
用于400次检测:在使用前不久,将全部体积的催化剂(1 ml)加入至全部体积的
染料溶液(45 ml)并充分混匀。
注意:反应混合物不应当进行储备;在立即使用前进行制备。
储备条件(工作溶液):2至8°C
冻干酶可在2至8°C稳定
重悬的催化剂溶液可在2至8°C稳定储存四周
一旦解冻,染料溶液可在2至8°C稳定储存数周。
用1 ml双蒸水将冻干酶重悬10分钟并充分混匀。
瓶2染料溶液:
即用型溶液。
反应混合物
用于100次检测:在使用前不久,将瓶1中的250 μl与瓶2中的11.25 ml的进行混合。
用于400次检测:在使用前不久,将全部体积的催化剂(1 ml)加入至全部体积的
染料溶液(45 ml)并充分混匀。
注意:反应混合物不应当进行储备;在立即使用前进行制备。
储备条件(工作溶液):2至8°C
冻干酶可在2至8°C稳定
重悬的催化剂溶液可在2至8°C稳定储存四周
一旦解冻,染料溶液可在2至8°C稳定储存数周。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
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商品
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
用于维持高质量经验证的无污染细胞培养的质量控制指南。免费下载ECACC手册。
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