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Roche

KAPA PROBE FAST

Name: KAPA PROBE FAST
Brand: ROCHE

suitable for qPCR, 2 ×, Universal

别名:

qPCR

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NACRES:

NA.55

UNSPSC Code:

41106300

主要文件

查看所有文档

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usage

sufficient for 100 reactions, sufficient for 1000 reactions, sufficient for 500 reactions, sufficient for 5000 reactions

shelf life

≤12 mo.

feature

dNTPs included, hotstart

packaging

kit of 1 mL (100 x 20 μL rxn; KK4701), kit of 10 mL (1000 x 20 μL rxn; KK4703), kit of 5 mL (500 x 20 μL rxn; KK4702), kit of 50 mL (5000 x 20 μL rxn; KK4715)

manufacturer/tradename

Roche

concentration

2 ×

technique(s)

qPCR: suitable

input

purified DNA

compatibility

for use with Agilent Mx3000P, for use with Agilent Mx3005P, for use with Agilent Mx4000, for use with Applied Biosystems^® 5700, for use with Applied Biosystems^® 7000, for use with Applied Biosystems^® 7300, for use with Applied Biosystems^® 7500, for use with Applied Biosystems^® 7700, for use with Applied Biosystems^® 7900HT, for use with Applied Biosystems^® ViiA 7, for use with QuantStudio^™, for use with StepOne^™, for use with StepOnePlus^™

detection method

probe-based

storage temp.

−20°C

Quality Level

100

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General description

KAPA PROBE FAST qPCR Kits 为所有基于探针的 qPCR 应用提供快速且可重复的结果。试剂盒包含即用型预混液，可使用序列特异性荧光探针化学，包括水解探针（例如，TaqMan ^®）、FRET 探针和置换探针（例如，分子信标），实现高灵敏度和高准确度的实时 PCR。

Application

KAPA PROBE FAST可用于：

甲基化特异性实时定量PCR(qMSP)
基因分型
基因表达分析
多重qPCR和qRT-qPCR
SNP基因分型/等位基因辨别
芯片验证

Features and Benefits

准确区分等位基因：

杂合子和纯合子等位基因的异常分化
SNP 基因分型检测中的离散簇

获得极佳的重现性和效率：

快速、高效五色多重 PCR
低拷贝编号时丰度水平相似
宽动态范围达 10 个数量级

增加灵活性：

与标准和快速循环方案兼容保留准确定量

快速注释：

这些试剂盒与所有基于荧光探针的技术兼容，包括杂交探针（例如，FRET）、水解探针（例如，TaqMan ^®）和置换探针（例如，分子信标）。
95ºC 下 20 s 的预变性足以进行酶再活化；然而，复杂靶标的最佳变性可能需要长达 3 min 的变性。
对于 2 步循环，使用 30 s 组合退火/延伸/数据采集。
对于 3 步循环，引物退火使用 20 s，延伸/数据采集使用 1 s。
不得超过 25μL 体积。

Preparation Note

ROX/荧光素引用染料（取决于试剂盒配置）对光敏感。长时间暴露于直射光下会导致荧光信号强度的损失。避免反复冻融。使用前务必确保产品已完全解冻并混合。

KAPA PROBE FAST qPCR Master Mix (2X) 可在 30 次冻融循环中保持稳定。确保所有试剂在不使用时均避光储存于-20°C 条件下。避光条件下，试剂在 4°C 避光条件下可稳定保存至少一周，只要不被微生物和/或核酸酶污染，可在此温度下短期使用。

Analysis Note

KAPA PROBE FAST qPCR Master Mix (2X) 经过严格的质量控制检测，不含污染的外切和核酸内切酶活性，并且符合 DNA 污染水平方面的严格要求。

Other Notes

仅用于研究目的。不用于诊断程序。

Legal Information

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

QuantStudio is a trademark of IROA Technologies LLC

StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

仅试剂盒组分

产品编号

说明

KAPA Taq HotStart^® DNA Polymerase

50X ROX^™ High Reference Dye

50X ROX^™ Low Reference Dye

reaction buffer

dNTPs

MgCl₂ 5 mM

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

常规特殊物品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species.

Holger Linck et al.

PloS one, 12(5), e0177808-e0177808 (2017-05-26)

Rubus stunt is an economically important disease in the production of raspberries, blackberries, and loganberries. A fast, sensitive, and reliable diagnosis of phytoplasmas, the causal agent of the disease, is of prime importance to stop its spread by vegetative propagation

Voltage-gated sodium channel polymorphisms play a pivotal role in the development of oxaliplatin-induced peripheral neurotoxicity: results from a prospective multicenter study.

Andreas A Argyriou et al.

Cancer, 119(19), 3570-3577 (2013-07-04)

The current prospective, multicenter study sought to identify single nucleotide polymorphisms of voltage-gated sodium channels (SCNAs) genes that might confer susceptibility to an increased incidence and severity of oxaliplatin-induced peripheral neuropathy (OXAIPN) in patients treated with either leucovorin, 5-fluorouracil, and

Molecular Analyses Define Vα7.2-Jα33+ MAIT Cell Depletion in HIV Infection: A Case-Control Study.

James E Ussher et al.

Medicine, 94(29), e1134-e1134 (2015-07-23)

Mucosal-associated invariant T (MAIT) cells are an abundant antibacterial innate-like lymphocyte population. There are conflicting reports as to their fate in HIV infection. The objective of this study was to determine whether MAIT cells are truly depleted in HIV infection.

Cell-free methylation markers with diagnostic and prognostic potential in hepatocellular carcinoma.

Lu C Y, et al.

Oncotarget, 8(4), 6406-6406 (2017)

Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression.

Peng W, et al.

Journal of Experimental & Clinical Cancer Research, 34(1), 79-79 (2015)

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KAPA PROBE FAST

suitable for qPCR, 2 ×, Universal

选择尺寸

关于此项目

主要文件

属性

usage

shelf life

feature

packaging

manufacturer/tradename

concentration

technique(s)

input

compatibility

detection method

storage temp.

Quality Level

相关类别

说明

General description

Application

Features and Benefits

Preparation Note

Analysis Note

Other Notes

Legal Information

仅试剂盒组分

安全信息

存储类别

wgk

flash_point_f

flash_point_c

法规信息

文件

分析证书(COA)

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