assay
>98% (HPLC and SDS-PAGE)
form
suspension
packaging
pkg of 15 mL (05015979001 [settled resin volume]), pkg of 2 mL (11719408001 [settled resin volume]), pkg of 5 mL (11134515001 [settled resin volume])
manufacturer/tradename
Roche
capacity
>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)
storage temp.
2-8°C
General description
重组蛋白A在蛋白A琼脂糖生产中的应用具有以下优点:它完全不含已知具有促有丝分裂作用的葡萄球菌肠毒素(分离自金黄色葡萄球菌的蛋白A中的污染物) 。固定化学可在广泛的pH和缓冲条件下提供高IgG的结合能力以及防漏稳定性。根据洗脱条件,凝胶可以使用30次以上。
蛋白A泄漏:<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98%纯度,HPLC,SDS-Page/1ml凝胶
蛋白A,固定化。
蛋白A泄漏:<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98%纯度,HPLC,SDS-Page/1ml凝胶
蛋白A,固定化。
Application
从腹水或细胞培养上清液中纯化小鼠单克隆抗体。蛋白A或蛋白G琼脂糖亲和基质可用于从粗细胞提取物中纯化抗体 以及蛋白质的免疫沉淀或共免疫沉淀。它还被用于光活化-核糖核苷-增强的交联免疫沉淀(PAR-CLIP)分析。
Biochem/physiol Actions
蛋白A是从特定细菌菌株中分离的细胞壁蛋白,其具有来自不同物种的某些类免疫球蛋白的特异性结合位点。蛋白A可在不同程度结合IgM、IgA、IgD和大多数IgG亚类。
Analysis Note
纯度:> 98%(HPLC,SDS-PAGE);完全不含葡萄球菌肠毒素。
Other Notes
Preswollen凝胶,即用型,2ml或5ml(沉淀凝胶体积)悬浮于缓冲液中的凝胶,该缓冲液中含有20%乙醇作为保藏剂,非无菌。
蛋白A含量:3mg/ml预溶胀凝胶
蛋白A含量:3mg/ml预溶胀凝胶
仅用于生命科学研究。不可用于诊断。
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signalword
Warning
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 1
flash_point_f
102.2 °F
flash_point_c
39 °C

