product line
BioReagent
assay
≥90% (HPLC), ≥90% (degree of coupling)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in methanol: water (1:1) (with 0.1% perchloric acid)
UV absorption
λ: 532-538 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
General description
Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.
Application
Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.
Features and Benefits
Characteristic features of the Atto 532 NHS ester are:
- Strong Absorption.
- High Fluorescence quantum yield.
- High Photostability.
- Excellent water solubility.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Rumelo Amor et al.
Scientific reports, 4, 7359-7359 (2014-12-09)
Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot laser scanning microscope by placing a
Kiyoto Kamagata et al.
Journal of the American Chemical Society, 134(28), 11525-11532 (2012-06-14)
A method was developed to detect fluorescence intensity signals from single molecules diffusing freely in a capillary cell. A unique optical system based on a spherical mirror was designed to enable quantitative detection of the fluorescence intensity. Furthermore, "flow-and-stop" control
Moritz Marcinowski et al.
Nature structural & molecular biology, 18(2), 150-158 (2011-01-11)
The endoplasmic reticulum is the site of folding, assembly and quality control for proteins of the secretory pathway. The ATP-regulated Hsp70 chaperone BiP (heavy chain-binding protein), together with cochaperones, has important roles in all of these processes. The functional cycle
John F Lesoine et al.
Nano letters, 12(6), 3273-3278 (2012-06-06)
We present a method for measuring the fluorescence from a single molecule hundreds of times without surface immobilization. The approach is based on the use of electroosmosis to repeatedly drive a single target molecule in a fused silica nanochannel through
Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binding.
Andrea Armbrüster et al.
FEBS letters, 579(9), 1961-1967 (2005-03-29)
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit
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