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Merck
CN

L5520

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Leibovitz L15

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Technical Service
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Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

shipped in

ambient

storage temp.

2-8°C

General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  
  • in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos
  • as a supplement in sterile cell media for the dissection and isolation of kidney cells
  • as a component of culture buffer for the tissue culture of Newt lung cells

Preparation Note

Supplement with 0.3 g/L L-glutamine.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

L-15 Media Formulation


Francisco J Monje et al.
Annals of medicine, 45(4), 301-307 (2013-05-02)
Studies of synaptic plasticity using the marine mollusk Aplysia californica as model system have been successfully used to identify proteins involved in learning and memory. The importance of molecular elements regulated by the learning- related neurotransmitter serotonin in Aplysia can
Daichi Kayo et al.
Scientific reports, 9(1), 8868-8868 (2019-06-22)
In vertebrates, sex steroids play crucial roles in multiple systems related to reproduction. In females, estrogens and their receptor estrogen receptor (ER or Esr) play indispensable roles in the negative sex steroid feedback regulation of pituitary gonadotropin secretion, which prevents
Applications of cell lines as bioreactors and in vitro models
Singh J and Goswami A
International journal of applied & basic medical research null



Global Trade Item Number

SKUGTIN
L5520-500ML04061833958223
L5520-100ML04061833958209
L5520-24X500ML04061833958216
L5520-6X500ML04061833958230