AL11430
DMT-locA(bz) Phosphoramidite
configured for ABI
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经验公式（希尔记法）:
C48H52N7O8P
化学文摘社编号:
分子量:
885.94
MDL number:
NACRES:
NA.51
UNSPSC Code:
41116105
产品名称
DMT-locA(bz) Phosphoramidite, configured for ABI
biological source
non-animal source (BSE/TSE no Risk)
product line
Proligo Reagents
assay
≥98.0% (31P-NMR)
≥98.0% (reversed phase HPLC)
form
powder
impurities
≤3 wt. % Residual Solvent Content
<0.4% Water Content (Karl Fischer)
<0.5% Single unspecified Impurity (reversed phase HPLC)
color
white to off-white
solubility
soluble, clear, colorless
absorption
<0.1 in acetonitrile at 0.2 M
suitability
conforms to structure for H-NMR
conforms to structure for LC-MS
compatibility
configured for ABI
storage temp.
−20°C
Quality Level
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Other Notes
LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran(THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.
Legal Information
Features and Benefits
Key Features of LNA Phosphoramidites :
- LNA oligonucleotides are prepared by phosphoramidite chemistry
- Standard DNA synthesizer platforms can be employed. No change is required in the reagents commonly used for DNA synthesis
- To further enhance the hybridization characteristics of LNA, 5-methyl-cytidine is employed instead of cytidine
- LNA monomers are as soluble in acetonitrile as their DNA counterparts (except for the 5-methyl-cytidine derivative, which requires the application of 10-20%, dichloromethane as a co-solvent)
- Mixmer oligonucleotides containing LNA, DNA and/or RNA monomers can be assembled easily
- LNA oligonucleotides with predefined melting temperatures (Tm) can be designed and prepared
General description
DMT-locA(bz) Phosphoramidite belongs to the group of LNA phosphoramidite. It is known as DMT-locAdenosine(N6-Benzoyl)- ß-Cyanoethylphosphoramidite. The synthesis cycle for Locked Nucleic Acid (LNA) oligonucleotides consists of the same series of reactions as the cycle that is employed for DNA monomers. However, the rate of coupling for LNA monomers is slower compared to that of DNA monomers (a coupling time of 8 minutes is recommended for LNA monomers compared to 90 seconds for DNA monomers).
With the exception of the LNA monomers, LNA synthesis is accomplished with the same reagents as DNA synthesis. LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran (THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.
With the exception of the LNA monomers, LNA synthesis is accomplished with the same reagents as DNA synthesis. LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran (THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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