C7089
Monoclonal Anti-Cdk7/CAK antibody produced in mouse
clone MO-1.1, ascites fluid
Product Name
Monoclonal Anti-Cdk7/CAK antibody produced in mouse, clone MO-1.1, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MO-1.1, monoclonal
mol wt
antigen 40 kDa
contains
15 mM sodium azide
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:50,000 using a cultured human cell line extract
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CDK7(1022)
Application
Monoclonal Anti-Cdk7/CAK antibody produced in mouse is suitable for the following applications:
- Immunocytochemistry
- Immunohistochemistry (frozen sections)
- Immunoprecipitation
- Microarray
- Western blotting at a dilution of 1:50,000 using a cultured human cell line extract
Biochem/physiol Actions
Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is involved in activation and phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle. It is required for cell cycle progression and it could be a target for cancer therapy. CAK plays a central role in regulation of messenger RNA synthesis by either inhibition of RNA polymerase II-catalyzed transcription or stimulation of transcription through association with basal transcription repair factor IIH. Phosphorylation by the CDK-activating kinase (CAK) is required for the activation of cyclin-dependent kinases.
The antibody reacts specifically with Cdk7 (40 kDa catalytic subunit of Cdk activating kinase).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cell division protein kinase 7 is an enzymes encoded by the CDK7 gene in humans. The protein belongs to cyclin-dependent protein kinase (CDK) family. CAK is a CDK-cyclin complex implicated in the control of multiple cell cycle transitions. The structure of CDK7 facilitates the inhibitors with differential specificity for the different CDKs and can be used in different biological processes.
Immunogen
recombinant human Cdk7 protein.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Stéphane Larochelle et al.
Methods in molecular biology (Clifton, N.J.), 296, 279-290 (2004-12-04)
All cyclin-dependent kinases (CDKs) involved in eukaryotic cell cycle control require phosphorylation at a conserved threonine (or serine) residue within the activation- or T-loop to attain full enzymatic activity. The enzyme responsible for this activating phosphorylation, the CDK-activating kinase (CAK)
Joel Rovnak et al.
Journal of virology, 86(10), 5742-5751 (2012-03-02)
Alterations in the functional levels of cyclin-dependent kinase-8 (CDK8) or its partner, cyclin C, have been clearly associated with cancers, including colon cancer, melanoma, and osteosarcoma. Walleye dermal sarcoma virus encodes a retroviral cyclin (RV-cyclin) that localizes to interchromatin granule
Graziano Lolli et al.
Cell cycle (Georgetown, Tex.), 4(4), 572-577 (2005-05-07)
The Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is responsible for the activating phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle. The kinase is composed of three subunits: CDK7, Cyclin H and MAT1 (ménage a trois).
D A Bochar et al.
The Journal of biological chemistry, 274(19), 13162-13166 (1999-05-01)
Cyclin-dependent kinase 7 (CDK7) can be isolated as a subunit of a trimeric kinase complex functional in activation of the mitotic promoting factor. In this study, we demonstrate that the trimeric cdk-activating kinase (CAK) acts as a transcriptional repressor of
R P Fisher et al.
Cell, 78(4), 713-724 (1994-08-26)
Phosphorylation by the CDK-activating kinase (CAK) is a required step in the activation of cyclin-dependent kinases. We have purified CAK from mammalian cells; the enzyme comprises two major polypeptides of 42 and 37 kDa. Protein sequencing indicates that the 42
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