产品名称
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L
shipped in
ambient
storage temp.
2-8°C
General description
在成分确定的培养基研究中，通常用成分确定的混合物（包括营养素、生长因子和激素）替代血清添加到培养基中。常用的组合是Dulbecco′s改良Eagle培养基(DME)与Ham′s F-12营养混合物（1:1混合）。为了补偿去血清后的缓冲能力损失，可在配方中添加15 mM终浓度的HEPES缓冲液，帮助研究人员优化缓冲体系。
Application
Dulbecco′s改良Eagle培养基/Ham F-12营养混合物已用于：
- 维持人肺和结直肠癌细胞系生存，进行次级代谢产物的生物学评估
- 培养人肾-2细胞(HK-2)并作为检测培养基组分，用于溶血磷脂酰胆碱(LPC)处理
- 小鼠鳞状细胞癌(SCCVII)肿瘤细胞培养
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Nutrient Mixture F-12 Ham Formulation
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
A new macrolide isolated from the endophytic fungus Colletotrichum sp
Bungihan ME, et al.
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Hayato Fukusumi et al.
PeerJ, 6, e4187-e4187 (2018-01-10)
Since the development of human-induced pluripotent stem cells (hiPSCs), various types of hiPSC-derived cells have been established for regenerative medicine and drug development. Neural stem/progenitor cells (NSPCs) derived from hiPSCs (hiPSC-NSPCs) have shown benefits for regenerative therapy of the central
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SLAS discovery : advancing life sciences R & D, 22(8), 974-984 (2017-06-24)
Polycystic kidney disease (PKD) is a prevalent disorder characterized by renal cysts that lead to kidney failure. Various signaling pathways have been targeted to stop disease progression, but most interventions still focus on alleviating PKD-associated symptoms. The mechanistic complexity of
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D8062-6X500ML | 04061833589182 |
| D8062-500ML | 04061833589175 |