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Merck
CN

D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

别名:

DME/F-12, 1:1混合

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
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产品名称

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

shipped in

ambient

storage temp.

2-8°C

General description

在成分确定的培养基研究中,通常用成分确定的混合物(包括营养素、生长因子和激素)替代血清添加到培养基中。常用的组合是Dulbecco′s改良Eagle培养基(DME)与Ham′s F-12营养混合物(1:1混合)。为了补偿去血清后的缓冲能力损失,可在配方中添加15 mM终浓度的HEPES缓冲液,帮助研究人员优化缓冲体系。

Application

Dulbecco′s改良Eagle培养基/Ham F-12营养混合物已用于:
  • 维持人肺和结直肠癌细胞系生存,进行次级代谢产物的生物学评估
  • 培养人肾-2细胞(HK-2)并作为检测培养基组分,用于溶血磷脂酰胆碱(LPC)处理
  • 小鼠鳞状细胞癌(SCCVII)肿瘤细胞培养



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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Nutrient Mixture F-12 Ham Formulation

钙是一种离子稳定的二价阳离子,在细胞培养基中具有有益和有毒的双重属性,可作为一系列细胞培养基的组成部分。


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全球贸易项目编号

货号GTIN
D8062-6X500ML04061833589182
D8062-500ML04061833589175