G5166
PNGase F Glycosidase
Removes N-linked glycans, suitable for mass spectrometry, from Elizabethkingia miricola
别名:
N-糖苷酶 F, 肽N-糖苷酶
产品名称
糖苷酶F from Elizabethkingia miricola, buffered aqueous solution
biological source
bacterial (Elizabethkingia miricola)
conjugate
(N-linked)
form
buffered aqueous solution
specific activity
≥20000 units/mg protein
mol wt
36 kDa
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
PNGase F（来自米尔伊丽莎白菌）已用于蛋白的去糖基化。
用于使蛋白质脱糖基。
Biochem/physiol Actions
如果糖基化天冬酰胺基团在其氨基和羧基端被多肽链取代，则从糖蛋白中切割整个聚糖。
General description
PNGase F（肽N-糖苷酶F)剪切天冬酰胺连接糖蛋白，生成无糖肽链和分离的全长寡糖。
Other Notes
一个单元将在37℃，pH7.5下通过SDS-PAGE监测，在1分钟内催化1纳摩尔变性核糖核酸酶B释放N-连接的寡糖。 PNGase F活性的一个 Sigma 单位等于1 IUB毫单位。
Physical form
以溶于 20 mM Tris HCl (pH 7.5)、50 mM NaCl 和 1 mM EDTA 的溶液形式提供
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
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Sun G, et al.
The Journal of Biological Chemistry, 290(12), 7452-7462 (2015)
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Lucy S Jun et al.
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商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
相关内容
Instructions
Glycobiology and Glycoproteomics Brochure
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