产品名称
Eagle最低必需培养基, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
最低必需培养基（MEM）是使用最广泛的合成细胞培养基之一。它包含更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。MEM已用于在胎牛血清（FBS）、小牛或马血清环境中培养各种单层细胞和附着细胞系（如成纤维细胞）。
Application
Eagle最低必需培养基已用于培养：
- 主动脉瓣小叶的外植体
- 人肝癌细胞系（HepG2）细胞
- 来源于人胎儿大脑祖细胞的星形胶质细胞（PDA）
- 乳腺癌细胞系(MCF-7)
- 人成胶质细胞瘤细胞系(T98G)
Preparation Note
补充0.292 g/L L-谷氨酰胺。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable