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M7167

M2 培养基

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

别名:

M2培养基溶液, 细胞培养 M2, 细胞培养基

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00283761
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Quality Level

300

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

shipped in

ambient

storage temp.

2-8°C

Application

推荐用于室温下操作小鼠胚胎。M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的Krebs-Ringer碳酸氢盐溶液，与Whitten′s培养基非常相似。M16含有丙酮酸和乳酸作为能量来源，因为植入前胚胎不能有效利用葡萄糖。

M2培养基是对M16的进一步改良，用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。

Preparation Note

添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。

Analysis Note

溶液中的沉淀不影响本品性能。
该产品经过测试，能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80％。

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动物来源培养基

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000425318
0000425317
0000425318
0000425317
0000450067

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商品

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

胚胎培养基

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

相关内容

Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Ludwig Stenz et al.
PloS one, 14(6), e0208371-e0208371 (2019-06-11)
Di(2-ethylhexyl)phthalate (DEHP) interferes with sex hormones signaling pathways (SHP). C57BL/6J mice prenatally exposed to 300 mg/kg/day DEHP develop a testicular dysgenesis syndrome (TDS) at adulthood, but similarly-exposed FVB/N mice are not affected. Here we aim to understand the reasons behind
Yi-Wen Xu et al.
Journal of cell science, 130(19), 3297-3307 (2017-08-19)
Precise regulation of DNA replication and genome integrity is crucial for gametogenesis and early embryogenesis. Cullin ring-finger ubiquitin ligase 4 (CRL4) has multiple functions in the maintenance of germ cell survival, oocyte meiotic maturation, and maternal-zygotic transition in mammals. DDB1-cullin-4-associated
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全球贸易项目编号

货号GTIN
M7167-50ML04061834061687
M7167-100ML04061838259820