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Merck
CN

M8823

Millipore

抗-FLAG® M2磁珠

affinity isolated antibody

别名:

单克隆抗-FLAG® M2 小鼠抗, FLAG®树脂，用于高通量, FLAG®磁性亲和树脂, Flag®亲和树脂, 抗 ddddk, 抗 dykddddk

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关于此项目

UNSPSC代码:
12352203
NACRES:
NA.32

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偶联物

magnetic beads

质量水平

200

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

M2, monoclonal

表单

suspension

保质期

2 yr at -20 °C

分析物化学类别

proteins

技术

affinity chromatography: suitable
immunoprecipitation (IP): suitable

珠子尺寸

20-75 μm

基质

superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.

同位素/亚型

IgG1

容量

≥0.6 mg/mL binding capacity

运输

wet ice

储存温度

−20°C

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相关类别

一般描述

抗-FLAG M2磁珠是可与抗-FLAG M2（小鼠单克隆）抗体结合的4％琼脂糖微珠。 该M2抗体可识别FLAG序列的N-末端、Met N-末端和C-末端。 这可实现对含有FLAG肽序列的融合蛋白的检测和捕获。

应用

适用免疫沉淀分析。

洗脱方法：FLAG®肽、甘氨酸(pH 3.5)、3x FLAG®肽

更多产品信息请访问我们的FLAG®应用页面。

特点和优势

该磁性属性可实现：
- 极为快速的分离
- 显著被加速的操作，例如重复的洗涤
-以板式进行多个样本的处理
该可导致：
- 更为快速的实验
- 更好的可重复性
- 目标蛋白更为准确的定量

外形

以含10mM磷酸钠、150mM 氯化钠（pH 7.4）、 0.02%（w/v）叠氮化钠（PBA/A）的50%甘油的50%悬浮液供应

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

FLAG®亲和凝胶、FLAG® 标签、3x FLAG®标签、DYKDDDDK标签

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000502258
0000502258
0000496134
0000496134
0000485683

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商品

ANTI-FLAG® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

ANTI-FLAG® M2磁珠

FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。

Anti-FLAG® M2磁珠

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

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