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MP0030

LookOut® Mycoplasma Elimination Kit

Synonym(s):

Mycoplasma contamination treatment

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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usage

 kit sufficient for 5 mL mycoplasma elimination

Quality Level

200

packaging

pkg of 1 kit

storage condition

protect from light

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

General description

The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.

Application

The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.
The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.

Features and Benefits

  • Minimal cytotoxic properties of the reagents
  • Highly effective
  • Retention of normal cell characteristics

Legal Information

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0000464755
0000464755
0000464760
0000464760
0000356675

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Protocols

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Articles

Mycoplasma Detection, Prevention, and Elimination in Cell Culture

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Why Use Antibiotics in Cell Culture?

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

View All Articles
Optimized PCR-based Detection of Mycoplasma
Dobrovolny PL and Bess D
Journal of Visualized Experiments, 52 (2011)

Global Trade Item Number

SKUGTIN
MP0030-1KT04061834066828